BRENDA - Enzyme Database show

Purification and biochemical characterization of the F1-ATPase from Acidithiobacillus ferrooxidans NASF-1 and analysis of the atp operon

Wakai, S.; Ohmori, A.; Kanao, T.; Sugio, T.; Kamimura, K.; Biosci. Biotechnol. Biochem. 69, 1884-1891 (2005)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
7.1.2.2
N,N'-dicyclohexylcarbodiimide
0.1 mM increases ATPase activity by 20%
Acidithiobacillus ferrooxidans
7.1.2.2
sulfite
50 mM stimulates 4fold
Acidithiobacillus ferrooxidans
Cloned(Commentary)
EC Number
Commentary
Organism
7.1.2.2
strain ATCC3020 in Escherichia coli-derived in vitro transcription-translation system
Acidithiobacillus ferrooxidans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
7.1.2.2
azide
2 mM inhibits 91% ATPase activity
Acidithiobacillus ferrooxidans
7.1.2.2
Cl-
-
Acidithiobacillus ferrooxidans
7.1.2.2
Cu2+
1 mM reduces ATPase activity 99% in the presence of 5 mM MgSO4
Acidithiobacillus ferrooxidans
7.1.2.2
Mn2+
1 mM reduces ATPase activity 50% in the presence of 5 mM MgSO4
Acidithiobacillus ferrooxidans
7.1.2.2
additional information
relatively resistant to vanadate, N,N'-dicyclohexylcarbodiimide and nitrate, 1 mM Fe3+, Ca2+ and Na+ do not affect ATPase activity in the presence of 5 mM MgSO4
Acidithiobacillus ferrooxidans
7.1.2.2
NaCl
inhibits ATPase activity
Acidithiobacillus ferrooxidans
7.1.2.2
Ni2+
1 mM reduces ATPase activity 47% in the presence of 5 mM MgSO4
Acidithiobacillus ferrooxidans
7.1.2.2
sulfate
slightly inhibits
Acidithiobacillus ferrooxidans
7.1.2.2
Zn2+
1 mM reduces ATPase activity 80% in the presence of 5 mM MgSO4
Acidithiobacillus ferrooxidans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7.1.2.2
1.8
-
ATP
estimated at pH 8.5 at 40C
Acidithiobacillus ferrooxidans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.1.2.2
membrane
-
Acidithiobacillus ferrooxidans
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
7.1.2.2
Mg2+
-
Acidithiobacillus ferrooxidans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.1.2.2
Acidithiobacillus ferrooxidans
-
strain NASF-1, strain ATCC33020
-
Purification (Commentary)
EC Number
Commentary
Organism
7.1.2.2
51fold by chromatographies, purified ATPase does not contain the F0-portion
Acidithiobacillus ferrooxidans
Storage Stability
EC Number
Storage Stability
Organism
7.1.2.2
4C, Tris-SO4 buffer, pH 8.5, 8 days
Acidithiobacillus ferrooxidans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.1.2.2
ATP + H2O + H+/in
-
672662
Acidithiobacillus ferrooxidans
ADP + phosphate + H+/out
-
-
-
?
7.1.2.2
GTP + H2O + H+/in
-
672662
Acidithiobacillus ferrooxidans
GDP + phosphate + H+/out
-
-
-
?
7.1.2.2
ITP + H2O + H+/in
-
672662
Acidithiobacillus ferrooxidans
IDP + phosphate + H+/out
-
-
-
?
7.1.2.2
additional information
no hydrolysis of UTP nor ADP
672662
Acidithiobacillus ferrooxidans
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
7.1.2.2
More
x * F1alpha, 55000 + x * F1beta, 50000, + x * F1gamma, 33000, + x * F1delta, 20000, + x * F1epsilon, 18000, SDS-PAGE of strain NASF-1, x * F1alpha, 55000 + x * F1beta, 50000, + x * F1gamma, 30000, + x * F1delta, 23000, + x * F1epsilon, 14000, SDS-PAGE of strain ATCC33020, x * F1alpha, 55500 + x * F1beta, 50500, + x * F1gamma, 33100, + x * F1delta, 19200, + x * F1epsilon, 15100, sequence analysis of strain NASF-1
Acidithiobacillus ferrooxidans
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
7.1.2.2
45
-
-
Acidithiobacillus ferrooxidans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.1.2.2
8.5
-
-
Acidithiobacillus ferrooxidans
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
7.1.2.2
7
9.5
ATPase activity decreases markedly above or below this range
Acidithiobacillus ferrooxidans
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
7.1.2.2
N,N'-dicyclohexylcarbodiimide
0.1 mM increases ATPase activity by 20%
Acidithiobacillus ferrooxidans
7.1.2.2
sulfite
50 mM stimulates 4fold
Acidithiobacillus ferrooxidans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
7.1.2.2
strain ATCC3020 in Escherichia coli-derived in vitro transcription-translation system
Acidithiobacillus ferrooxidans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
7.1.2.2
azide
2 mM inhibits 91% ATPase activity
Acidithiobacillus ferrooxidans
7.1.2.2
Cl-
-
Acidithiobacillus ferrooxidans
7.1.2.2
Cu2+
1 mM reduces ATPase activity 99% in the presence of 5 mM MgSO4
Acidithiobacillus ferrooxidans
7.1.2.2
Mn2+
1 mM reduces ATPase activity 50% in the presence of 5 mM MgSO4
Acidithiobacillus ferrooxidans
7.1.2.2
additional information
relatively resistant to vanadate, N,N'-dicyclohexylcarbodiimide and nitrate, 1 mM Fe3+, Ca2+ and Na+ do not affect ATPase activity in the presence of 5 mM MgSO4
Acidithiobacillus ferrooxidans
7.1.2.2
NaCl
inhibits ATPase activity
Acidithiobacillus ferrooxidans
7.1.2.2
Ni2+
1 mM reduces ATPase activity 47% in the presence of 5 mM MgSO4
Acidithiobacillus ferrooxidans
7.1.2.2
sulfate
slightly inhibits
Acidithiobacillus ferrooxidans
7.1.2.2
Zn2+
1 mM reduces ATPase activity 80% in the presence of 5 mM MgSO4
Acidithiobacillus ferrooxidans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
7.1.2.2
1.8
-
ATP
estimated at pH 8.5 at 40C
Acidithiobacillus ferrooxidans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.1.2.2
membrane
-
Acidithiobacillus ferrooxidans
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
7.1.2.2
Mg2+
-
Acidithiobacillus ferrooxidans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
7.1.2.2
51fold by chromatographies, purified ATPase does not contain the F0-portion
Acidithiobacillus ferrooxidans
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
7.1.2.2
4C, Tris-SO4 buffer, pH 8.5, 8 days
Acidithiobacillus ferrooxidans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.1.2.2
ATP + H2O + H+/in
-
672662
Acidithiobacillus ferrooxidans
ADP + phosphate + H+/out
-
-
-
?
7.1.2.2
GTP + H2O + H+/in
-
672662
Acidithiobacillus ferrooxidans
GDP + phosphate + H+/out
-
-
-
?
7.1.2.2
ITP + H2O + H+/in
-
672662
Acidithiobacillus ferrooxidans
IDP + phosphate + H+/out
-
-
-
?
7.1.2.2
additional information
no hydrolysis of UTP nor ADP
672662
Acidithiobacillus ferrooxidans
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
7.1.2.2
More
x * F1alpha, 55000 + x * F1beta, 50000, + x * F1gamma, 33000, + x * F1delta, 20000, + x * F1epsilon, 18000, SDS-PAGE of strain NASF-1, x * F1alpha, 55000 + x * F1beta, 50000, + x * F1gamma, 30000, + x * F1delta, 23000, + x * F1epsilon, 14000, SDS-PAGE of strain ATCC33020, x * F1alpha, 55500 + x * F1beta, 50500, + x * F1gamma, 33100, + x * F1delta, 19200, + x * F1epsilon, 15100, sequence analysis of strain NASF-1
Acidithiobacillus ferrooxidans
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
7.1.2.2
45
-
-
Acidithiobacillus ferrooxidans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.1.2.2
8.5
-
-
Acidithiobacillus ferrooxidans
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
7.1.2.2
7
9.5
ATPase activity decreases markedly above or below this range
Acidithiobacillus ferrooxidans