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Literature summary extracted from

  • Wakai, S.; Ohmori, A.; Kanao, T.; Sugio, T.; Kamimura, K.
    Purification and biochemical characterization of the F1-ATPase from Acidithiobacillus ferrooxidans NASF-1 and analysis of the atp operon (2005), Biosci. Biotechnol. Biochem., 69, 1884-1891.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.1.2.2 N,N'-dicyclohexylcarbodiimide 0.1 mM increases ATPase activity by 20% Acidithiobacillus ferrooxidans
7.1.2.2 sulfite 50 mM stimulates 4fold Acidithiobacillus ferrooxidans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.2.2 strain ATCC3020 in Escherichia coli-derived in vitro transcription-translation system Acidithiobacillus ferrooxidans

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.2.2 azide 2 mM inhibits 91% ATPase activity Acidithiobacillus ferrooxidans
7.1.2.2 Cl-
-
 Acidithiobacillus ferrooxidans
7.1.2.2 Cu2+ 1 mM reduces ATPase activity 99% in the presence of 5 mM MgSO4 Acidithiobacillus ferrooxidans
7.1.2.2 Mn2+ 1 mM reduces ATPase activity 50% in the presence of 5 mM MgSO4 Acidithiobacillus ferrooxidans
7.1.2.2 additional information relatively resistant to vanadate, N,N'-dicyclohexylcarbodiimide and nitrate, 1 mM Fe3+, Ca2+ and Na+ do not affect ATPase activity in the presence of 5 mM MgSO4 Acidithiobacillus ferrooxidans
7.1.2.2 NaCl inhibits ATPase activity Acidithiobacillus ferrooxidans
7.1.2.2 Ni2+ 1 mM reduces ATPase activity 47% in the presence of 5 mM MgSO4 Acidithiobacillus ferrooxidans
7.1.2.2 sulfate slightly inhibits Acidithiobacillus ferrooxidans
7.1.2.2 Zn2+ 1 mM reduces ATPase activity 80% in the presence of 5 mM MgSO4 Acidithiobacillus ferrooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.2.2 1.8
-
 ATP estimated at pH 8.5 at 40°C Acidithiobacillus ferrooxidans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.2.2 membrane
-
 Acidithiobacillus ferrooxidans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.2.2 Mg2+
-
 Acidithiobacillus ferrooxidans

Organism

EC Number Organism UniProt Comment Textmining
7.1.2.2 Acidithiobacillus ferrooxidans
-
 strain NASF-1, strain ATCC33020
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.2.2 51fold by chromatographies, purified ATPase does not contain the F0-portion Acidithiobacillus ferrooxidans

Storage Stability

EC Number Storage Stability Organism
7.1.2.2 4°C, Tris-SO4 buffer, pH 8.5, 8 days Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.2.2 ATP + H2O + H+/in
-
 Acidithiobacillus ferrooxidans ADP + phosphate + H+/out
-
 ?
7.1.2.2 GTP + H2O + H+/in
-
 Acidithiobacillus ferrooxidans GDP + phosphate + H+/out
-
 ?
7.1.2.2 ITP + H2O + H+/in
-
 Acidithiobacillus ferrooxidans IDP + phosphate + H+/out
-
 ?
7.1.2.2 additional information no hydrolysis of UTP nor ADP Acidithiobacillus ferrooxidans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.2.2 More x * F1alpha, 55000 + x * F1beta, 50000, + x * F1gamma, 33000, + x * F1delta, 20000, + x * F1epsilon, 18000, SDS-PAGE of strain NASF-1, x * F1alpha, 55000 + x * F1beta, 50000, + x * F1gamma, 30000, + x * F1delta, 23000, + x * F1epsilon, 14000, SDS-PAGE of strain ATCC33020, x * F1alpha, 55500 + x * F1beta, 50500, + x * F1gamma, 33100, + x * F1delta, 19200, + x * F1epsilon, 15100, sequence analysis of strain NASF-1 Acidithiobacillus ferrooxidans

Synonyms

EC Number Synonyms Comment Organism
7.1.2.2 F1-ATPase
-
 Acidithiobacillus ferrooxidans
7.1.2.2 F1F0-ATPase
-
 Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.1.2.2 45
-
-
 Acidithiobacillus ferrooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.2.2 8.5
-
-
 Acidithiobacillus ferrooxidans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
7.1.2.2 7 9.5 ATPase activity decreases markedly above or below this range Acidithiobacillus ferrooxidans