Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Khan, I.H.; Kim, H.; Ashida, H.; Ishikawa, T.; Shibata, H.; Sawa, Y.
    Altering the substrate specificity of glutamate dehydrogenase from Bacillus subtilis by site-directed mutagenesis (2005), Biosci. Biotechnol. Biochem., 69, 1802-1805.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.2 expression in Escherichia coli Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.2 G82K dramatically switches to increased specificity for oxaloacetate, 280fold higher than those for 2-oxoglutarate Bacillus subtilis
1.4.1.2 G82R specific activity not drastically altered compared to the wild-type Bacillus subtilis
1.4.1.2 K80R specific activity not drastically altered compared to the wild-type Bacillus subtilis
1.4.1.2 M101K specific activity not drastically altered compared to the wild-type Bacillus subtilis
1.4.1.2 M101S dramatically switches to increased specificity for oxaloacetate, 495fold higher than those for 2-oxoglutarate Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.2 0.07
-
NADH wild-type Bacillus subtilis
1.4.1.2 0.091
-
NADH mutant M101S Bacillus subtilis
1.4.1.2 0.095
-
NADH mutant G82K Bacillus subtilis
1.4.1.2 0.65
-
2-oxoglutarate wild-type Bacillus subtilis
1.4.1.2 2.27
-
oxaloacetate mutant M101S Bacillus subtilis
1.4.1.2 4.16
-
oxaloacetate mutant G82K Bacillus subtilis
1.4.1.2 100
-
2-oxoglutarate mutant M101S Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.2 46000
-
SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.2 to homogeneity Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.2 0.012
-
2-oxoglutarate, mutant M101K Bacillus subtilis
1.4.1.2 0.014
-
2-oxoglutarate, mutant K80R Bacillus subtilis
1.4.1.2 0.019
-
2-oxoglutarate, mutant G82R and M101S Bacillus subtilis
1.4.1.2 0.026
-
2-oxoglutarate, mutant G82K Bacillus subtilis
1.4.1.2 0.21
-
on L-aspartate, mutant M101S Bacillus subtilis
1.4.1.2 0.39
-
on L-aspartate, mutant G82K Bacillus subtilis
1.4.1.2 440
-
2-oxoglutarate, wild-type Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 2-oxo-3-methylvalerate + NADPH + NH3 very low specificity of wild-type Bacillus subtilis L-isoleucine + NADP+ + H2O
-
?
1.4.1.2 2-oxo-iso-caproate + NADPH + NH3 very low specificity of wild-type Bacillus subtilis L-norleucine + NADP+ + H2O
-
?
1.4.1.2 2-oxo-iso-valerate + NADPH + NH3 low specificity of wild-type Bacillus subtilis L-leucine + NADP+ + H2O
-
?
1.4.1.2 2-oxoglutarate + NADPH + NH3 wild-type enzyme highly specific for 2-oxoglutarate Bacillus subtilis L-glutamate + NADP+ + H2O
-
r
1.4.1.2 beta-phenylpyruvate + NADPH + NH3 low specificity of wild-type Bacillus subtilis L-phenylalanine + NADP+ + H2O
-
?
1.4.1.2 additional information no specificity of wild-type for 2-ketohexonoate Bacillus subtilis ?
-
?
1.4.1.2 oxaloacetate + NADPH + NH3 very low specificity of wild-type Bacillus subtilis L-aspartate + NADP+ + H2O
-
?
1.4.1.2 p-hydroxyphenylpyruvate + NADPH + NH3 very low specificity of wild-type Bacillus subtilis L-tyrosine + NADP+ + H2O
-
?
1.4.1.2 pyruvate + NADPH + NH3 very low specificity of wild-type Bacillus subtilis L-alanine + NADP+ + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.2 glutamate dehydrogenase
-
Bacillus subtilis
1.4.1.2 NAD+-dependent GluDH
-
Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.2 additional information
-
oxaloacetate mutant M101S Bacillus subtilis
1.4.1.2 0.012
-
2-oxoglutarate mutant M101S Bacillus subtilis
1.4.1.2 0.013
-
2-oxoglutarate mutant G82K Bacillus subtilis
1.4.1.2 3.45
-
oxaloacetate mutant G82K Bacillus subtilis
1.4.1.2 5.68
-
oxaloacetate mutant M101S Bacillus subtilis
1.4.1.2 344
-
2-oxoglutarate wild-type Bacillus subtilis