EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.1.2 | expression in Escherichia coli | Bacillus subtilis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.1.2 | G82K | dramatically switches to increased specificity for oxaloacetate, 280fold higher than those for 2-oxoglutarate | Bacillus subtilis |
1.4.1.2 | G82R | specific activity not drastically altered compared to the wild-type | Bacillus subtilis |
1.4.1.2 | K80R | specific activity not drastically altered compared to the wild-type | Bacillus subtilis |
1.4.1.2 | M101K | specific activity not drastically altered compared to the wild-type | Bacillus subtilis |
1.4.1.2 | M101S | dramatically switches to increased specificity for oxaloacetate, 495fold higher than those for 2-oxoglutarate | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.2 | 0.07 | - |
NADH | wild-type | Bacillus subtilis | |
1.4.1.2 | 0.091 | - |
NADH | mutant M101S | Bacillus subtilis | |
1.4.1.2 | 0.095 | - |
NADH | mutant G82K | Bacillus subtilis | |
1.4.1.2 | 0.65 | - |
2-oxoglutarate | wild-type | Bacillus subtilis | |
1.4.1.2 | 2.27 | - |
oxaloacetate | mutant M101S | Bacillus subtilis | |
1.4.1.2 | 4.16 | - |
oxaloacetate | mutant G82K | Bacillus subtilis | |
1.4.1.2 | 100 | - |
2-oxoglutarate | mutant M101S | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.2 | 46000 | - |
SDS-PAGE | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.2 | Bacillus subtilis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.2 | to homogeneity | Bacillus subtilis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.2 | 0.012 | - |
2-oxoglutarate, mutant M101K | Bacillus subtilis |
1.4.1.2 | 0.014 | - |
2-oxoglutarate, mutant K80R | Bacillus subtilis |
1.4.1.2 | 0.019 | - |
2-oxoglutarate, mutant G82R and M101S | Bacillus subtilis |
1.4.1.2 | 0.026 | - |
2-oxoglutarate, mutant G82K | Bacillus subtilis |
1.4.1.2 | 0.21 | - |
on L-aspartate, mutant M101S | Bacillus subtilis |
1.4.1.2 | 0.39 | - |
on L-aspartate, mutant G82K | Bacillus subtilis |
1.4.1.2 | 440 | - |
2-oxoglutarate, wild-type | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.2 | 2-oxo-3-methylvalerate + NADPH + NH3 | very low specificity of wild-type | Bacillus subtilis | L-isoleucine + NADP+ + H2O | - |
? | |
1.4.1.2 | 2-oxo-iso-caproate + NADPH + NH3 | very low specificity of wild-type | Bacillus subtilis | L-norleucine + NADP+ + H2O | - |
? | |
1.4.1.2 | 2-oxo-iso-valerate + NADPH + NH3 | low specificity of wild-type | Bacillus subtilis | L-leucine + NADP+ + H2O | - |
? | |
1.4.1.2 | 2-oxoglutarate + NADPH + NH3 | wild-type enzyme highly specific for 2-oxoglutarate | Bacillus subtilis | L-glutamate + NADP+ + H2O | - |
r | |
1.4.1.2 | beta-phenylpyruvate + NADPH + NH3 | low specificity of wild-type | Bacillus subtilis | L-phenylalanine + NADP+ + H2O | - |
? | |
1.4.1.2 | additional information | no specificity of wild-type for 2-ketohexonoate | Bacillus subtilis | ? | - |
? | |
1.4.1.2 | oxaloacetate + NADPH + NH3 | very low specificity of wild-type | Bacillus subtilis | L-aspartate + NADP+ + H2O | - |
? | |
1.4.1.2 | p-hydroxyphenylpyruvate + NADPH + NH3 | very low specificity of wild-type | Bacillus subtilis | L-tyrosine + NADP+ + H2O | - |
? | |
1.4.1.2 | pyruvate + NADPH + NH3 | very low specificity of wild-type | Bacillus subtilis | L-alanine + NADP+ + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.1.2 | glutamate dehydrogenase | - |
Bacillus subtilis |
1.4.1.2 | NAD+-dependent GluDH | - |
Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.2 | additional information | - |
oxaloacetate | mutant M101S | Bacillus subtilis | |
1.4.1.2 | 0.012 | - |
2-oxoglutarate | mutant M101S | Bacillus subtilis | |
1.4.1.2 | 0.013 | - |
2-oxoglutarate | mutant G82K | Bacillus subtilis | |
1.4.1.2 | 3.45 | - |
oxaloacetate | mutant G82K | Bacillus subtilis | |
1.4.1.2 | 5.68 | - |
oxaloacetate | mutant M101S | Bacillus subtilis | |
1.4.1.2 | 344 | - |
2-oxoglutarate | wild-type | Bacillus subtilis |