Literature summary extracted from
Ung, M.; Lu, B.; McCammon, J.A.
E230Q mutation of the catalytic subunit of cAMP-dependent protein kinase affects local structure and the binding of peptide inhibitor (2006), Biopolymers, 81, 428-439.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.7.11.11 |
crystallization of the muatnt E230Q with MgATP2- and protein kinase inhibitor is only possible as apoenzyme, analysis of the mechanism preventing ternary complex formation by relaxed-complex method |
Mus musculus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.7.11.11 |
E230Q |
site-directed mutagenesis of the catalytic subunit, the mutation affects the local structure of the the F-to-G helix loop and asp241, and the binding of protein kinase inhibitor PKI, the mutant protein can only be crystallizes as apoenzyme |
Mus musculus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.11.11 |
protein kinase inhibitor PKI |
binding structure, overview |
Mus musculus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.11.11 |
Mg2+ |
- |
Mus musculus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.11.11 |
Mus musculus |
P05132 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.11.11 |
ATP + kemptide |
- |
Mus musculus |
ADP + phospho-kemptide |
- |
? |
|
2.7.11.11 |
additional information |
the catalytic subunit has a cluster of nonconserved acidic residues, Glu127, Glu170, Glu203, Glu230, and Asp241, that are crucial for substrate recognition and binding |
Mus musculus |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.11.11 |
More |
the catalytic subunit has a cluster of nonconserved acidic residues, Glu127, Glu170, Glu203, Glu230, and Asp241, that are crucial for substrate recognition and binding, protein dynamics of the catalytic C-subunit, overview |
Mus musculus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.7.11.11 |
PKA |
- |
Mus musculus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.7.11.11 |
ATP |
- |
Mus musculus |
|