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Literature summary extracted from

  • Kharel, M.K.; Subba, B.; Lee, H.C.; Liou, K.; Sohng, J.K.
    Characterization of L-glutamine:2-deoxy-scyllo-inosose aminotransferase (tbmB) from Streptomyces tenebrarius (2005), Bioorg. Med. Chem. Lett., 15, 89-92.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.50 expressed heterologously in Escherichia coli Streptoalloteichus tenebrarius
2.6.1.100 gene tbmB, expression in Escherichia coli strain BL21(DE3) Streptoalloteichus tenebrarius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.50 2-deoxy-scyllo-inosose + L-glutamine Streptoalloteichus tenebrarius tbmB catalyzes the second step of the DOS biosynthetic pathway during the biosynthesis of 2-deoxystreptamine, a subunit of tobramycin, in Streptomyces tenebrarius 2-deoxy-scyllo-inosamine + 2-ketoglutaramate
-
 ?
2.6.1.100 L-glutamine + 2-deoxy-scyllo-inosose Streptoalloteichus tenebrarius
-
 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.50 Streptoalloteichus tenebrarius
-
-
-
2.6.1.100 Streptoalloteichus tenebrarius Q2MF17 gene tbmB; gene tbmB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.50 2-deoxy-scyllo-inosose + L-glutamine
-
 Streptoalloteichus tenebrarius 2-deoxy-scyllo-inosamine + 2-ketoglutaramate
-
 ?
2.6.1.50 2-deoxy-scyllo-inosose + L-glutamine tbmB catalyzes the second step of the DOS biosynthetic pathway during the biosynthesis of 2-deoxystreptamine, a subunit of tobramycin, in Streptomyces tenebrarius Streptoalloteichus tenebrarius 2-deoxy-scyllo-inosamine + 2-ketoglutaramate
-
 ?
2.6.1.100 L-glutamine + 2-deoxy-scyllo-inosose
-
 Streptoalloteichus tenebrarius 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
 ?
2.6.1.100 additional information coupled assays of TbmB with BtrC and myo-inositol dehydrogenase are performed Streptoalloteichus tenebrarius ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.50 tbmB
-
 Streptoalloteichus tenebrarius
2.6.1.100 2-deoxy-scyllo-inosose aminotransferase
-
 Streptoalloteichus tenebrarius
2.6.1.100 tbmB
-
 Streptoalloteichus tenebrarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.100 37
-
 assay at Streptoalloteichus tenebrarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.100 7.5
-
 assay at Streptoalloteichus tenebrarius

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.100 pyridoxal 5'-phosphate
-
 Streptoalloteichus tenebrarius

General Information

EC Number General Information Comment Organism
2.6.1.100 metabolism the enzyme is involved in tobramycin biosynthesis catalyzing the second step of the 2-deoxystreptamine biosynthetic pathway, 2-deoxystreptamine is a subunit of tobramycin, overview Streptoalloteichus tenebrarius