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Literature summary extracted from

  • Woo, Y.H.; Fernandes, R.P.; Proteau, P.J.
    Evaluation of fosmidomycin analogs as inhibitors of the Synechocystis sp. PCC6803 1-deoxy-D-xylulose 5-phosphate reductoisomerase (2006), Bioorg. Med. Chem., 14, 2375-2385.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.267 drug development the enzyme is a target for antibacterial agents and inhibitor design Synechocystis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.267 4-(N-formyl-N-hydroxy-amino)-butyric acid
-
 Synechocystis sp.
1.1.1.267 fosfoxacin phosphate analogue of fosmidomycin Synechocystis sp.
1.1.1.267 fosmidomycin
-
 Synechocystis sp.
1.1.1.267 FR900098 N-acetyl analogue of fosmidomycin Synechocystis sp.
1.1.1.267 phosphoric acid mono-[2-(N-acetyl-N-hydroxy-amino)-ethyl]-ester
-
 Synechocystis sp.
1.1.1.267 sulfamic acid 2-(N-formyl-N-hydroxy-amino)-ethyl ester
-
 Synechocystis sp.
1.1.1.267 [3-(N-acetyl-N-methyl-amino)propyl]-phosphonic acid
-
 Synechocystis sp.
1.1.1.267 [3-(N-formyl-N-methyl-amino)-propyl]-phosphonic acid
-
 Synechocystis sp.
1.1.1.267 [4-(hydroxyamino)-4-oxobutyl]phosphonic acid
-
 Synechocystis sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.267 Mg2+ a divalent metal ion is absolutely required for activity, involved in catalysis Synechocystis sp.
1.1.1.267 Mn2+ a divalent metal ion is absolutely required for activity, involved in catalysis Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Synechocystis sp. step in the methylerythritol 4-phosphate pathway 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Synechocystis sp.
-
 strain PCC6803
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.267 2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ reaction mechanism via 2-C-methylerythrose 4-phosphate intermediate Synechocystis sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ step in the methylerythritol 4-phosphate pathway Synechocystis sp. 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ i.e. DXP Synechocystis sp. 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 1-deoxy-D-xylulose 5-phosphate reductoisomerase
-
 Synechocystis sp.
1.1.1.267 DXR
-
 Synechocystis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.267 37
-
 assay at Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.267 7.5
-
 assay at Synechocystis sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADPH
-
 Synechocystis sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.267 0.000002
-
 phosphoric acid mono-[2-(N-acetyl-N-hydroxy-amino)-ethyl]-ester pH 7.5, 37°C Synechocystis sp.
1.1.1.267 0.000019
-
 fosfoxacin pH 7.5, 37°C Synechocystis sp.
1.1.1.267 0.000057
-
 fosmidomycin pH 7.5, 37°C Synechocystis sp.
1.1.1.267 0.0009
-
 FR900098 pH 7.5, 37°C Synechocystis sp.
1.1.1.267 0.24
-
 4-(N-formyl-N-hydroxy-amino)-butyric acid pH 7.5, 37°C Synechocystis sp.
1.1.1.267 2.4
-
 [4-(hydroxyamino)-4-oxobutyl]phosphonic acid pH 7.5, 37°C Synechocystis sp.
1.1.1.267 2.8
-
 sulfamic acid 2-(N-formyl-N-hydroxy-amino)-ethyl ester pH 7.5, 37°C Synechocystis sp.
1.1.1.267 3.6
-
 [3-(N-acetyl-N-methyl-amino)propyl]-phosphonic acid pH 7.5, 37°C Synechocystis sp.
1.1.1.267 5
-
 [3-(N-formyl-N-methyl-amino)-propyl]-phosphonic acid pH 7.5, 37°C Synechocystis sp.