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Literature summary extracted from

  • Ma, Q.H.; Tian, B.
    Biochemical characterization of a cinnamoyl-CoA reductase from wheat (2005), Biol. Chem., 386, 553-560.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.44 specific PCR product is cloned into the pGEM-T Easy vector, as an expression vector for Escherichia coli, pET28a is used Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.44 0.023
-
 feruloyl-CoA
-
 Triticum aestivum
1.2.1.44 0.0346
-
 5-hydroxyferuloyl-CoA
-
 Triticum aestivum
1.2.1.44 0.0366
-
 sinapoyl-CoA
-
 Triticum aestivum
1.2.1.44 0.0397
-
 4-coumaroyl-CoA
-
 Triticum aestivum
1.2.1.44 0.061
-
 caffeoyl-CoA
-
 Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.44 39420
-
 calculated molecular mass Triticum aestivum
1.2.1.44 50000
-
 SDS-PAGE Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.44 cinnamaldehyde + CoA + NADP+ Triticum aestivum
-
 cinnamoyl-CoA + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.44 Triticum aestivum Q4KUK8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.44 using Ni-NTA His-Bind resin Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.44 leaf
-
 Triticum aestivum
-
1.2.1.44 root
-
 Triticum aestivum
-
1.2.1.44 stem
-
 Triticum aestivum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.44 4-coumaroyl-CoA + NADPH + H+
-
 Triticum aestivum 4-coumaraldehyde + CoA + NADP+
-
 ?
1.2.1.44 5-hydroxyferuloyl-CoA + NADPH + H+
-
 Triticum aestivum 5-hydroxyconiferaldehyde + CoA + NADP+
-
 ?
1.2.1.44 caffeoyl-CoA + NADPH + H+
-
 Triticum aestivum caffealdehyde + CoA + NADP+
-
 ?
1.2.1.44 cinnamaldehyde + CoA + NADP+
-
 Triticum aestivum cinnamoyl-CoA + NADPH + H+
-
 ?
1.2.1.44 feruloyl-CoA + NADPH + H+
-
 Triticum aestivum coniferaldehyde + CoA + NADP+
-
 ?
1.2.1.44 sinapoyl-CoA + NADPH + H+
-
 Triticum aestivum sinapaldehyde + CoA + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.44 CCR ambiguous Triticum aestivum
1.2.1.44 cinnamoyl-CoA reductase
-
 Triticum aestivum
1.2.1.44 Ta-CCR2
-
 Triticum aestivum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.44 NADPH
-
 Triticum aestivum