Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bakke, M.; Setoyama, C.; Miura, R.; Kajiyama, N.
    N-Ethylmaleimide-resistant acyl-coenzyme A oxidase from Arthrobacter ureafaciens NBRC 12140: Molecular cloning, gene expression and characterization of the recombinant enzyme (2007), Biochim. Biophys. Acta, 1774, 65-71.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.3.6 analysis useful for the determination of free fatty acids Paenarthrobacter ureafaciens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.6 expressed in Escherichia coli JM109 cells Paenarthrobacter ureafaciens
1.3.3.6 into vector pUTE300, expression in Escherichia coli JM109 Paenarthrobacter ureafaciens
1.3.3.6 into vector pUTE300, expression in Escherichia coli JM109 Arthrobacter sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.6 C159T exhibits 60% activity of the wild-type enzyme, looses more than half of the activity after incubation with N-ethylmaleimide Paenarthrobacter ureafaciens
1.3.3.6 C159T 60% activity compared to the wild type enzyme and shows increased sensitivity to N-ethylmaleimide Paenarthrobacter ureafaciens
1.3.3.6 C159T/C420S activity less than one tenth of that of the wild type Paenarthrobacter ureafaciens
1.3.3.6 C159T/C420S/C424V shows no activity at all Paenarthrobacter ureafaciens
1.3.3.6 C159T/C424V activity less than one tenth of that of the wild type Paenarthrobacter ureafaciens
1.3.3.6 C420S exhibits 41% activity of the wild-type enzyme, retains about 90% of the activity after incubation with N-ethylmaleimide Paenarthrobacter ureafaciens
1.3.3.6 C420S 41% activity compared to the wild type enzyme and shows increased sensitivity to N-ethylmaleimide Paenarthrobacter ureafaciens
1.3.3.6 C420S/424V activity less than one tenth of that of the wild type Paenarthrobacter ureafaciens
1.3.3.6 C424V looses more than half of the activity after incubation with N-Ethylmaleimide Paenarthrobacter ureafaciens
1.3.3.6 C424V 98% activity compared to the wild type enzyme and shows increased sensitivity to N-ethylmaleimide Paenarthrobacter ureafaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.6 N-ethylmaleimide shows less than 10% activity in the presence of 10 mM at 37°C for 4 h Arthrobacter sp.
1.3.3.6 N-ethylmaleimide shows less than 10% activity in the presence of 10 mM at 37 °C for 4 h Candida sp. (in: Saccharomycetales)
1.3.3.6 N-ethylmaleimide ACO retains more than 60% of the initial activity in the presence of 10 mM N-ethylmaleimide, at 37°C for 4 h; purified enzyme retains more than 60% activity in the presence of 10 mM at 37°C for 4 h, while other commercially available ACOs show only less than 10% activities after the same treatment Paenarthrobacter ureafaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.6 0.00000002
-
 palmitoyl-CoA
-
 Paenarthrobacter ureafaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.6 75000
-
 2 * 75000, SDS-PAGE Paenarthrobacter ureafaciens
1.3.3.6 76400
-
 predicted Arthrobacter sp.
1.3.3.6 76500
-
 predicted from amino acid sequence Paenarthrobacter ureafaciens
1.3.3.6 95000
-
 2 * 95000, gel filtration Paenarthrobacter ureafaciens
1.3.3.6 160000
-
 native-PAGE Paenarthrobacter ureafaciens
1.3.3.6 180000
-
 SDS-PAGE, recombinant enzyme Paenarthrobacter ureafaciens
1.3.3.6 190000
-
 gel filtration Paenarthrobacter ureafaciens
1.3.3.6 190000
-
 gel filtration, recombinant enzyme Paenarthrobacter ureafaciens

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.6 Arthrobacter sp. Q33DR1 strain 2-4-1
-
1.3.3.6 Arthrobacter sp. 02.04.2001 Q33DR1 strain 2-4-1
-
1.3.3.6 Candida sp. (in: Saccharomycetales)
-
-
-
1.3.3.6 Paenarthrobacter ureafaciens Q33DR0
-
-
1.3.3.6 Paenarthrobacter ureafaciens NBRC 12140 Q33DR0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.6 by gel filtration Paenarthrobacter ureafaciens
1.3.3.6 Q-Sepharose column chromatography and CM-Sepharose column chromatography Paenarthrobacter ureafaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.6 1.7
-
 crude enzyme Paenarthrobacter ureafaciens
1.3.3.6 1.7
-
 crude extract, at 37°C Paenarthrobacter ureafaciens
1.3.3.6 60.9
-
 purified enzyme Paenarthrobacter ureafaciens
1.3.3.6 60.9
-
 purified recombinant enzyme, at 37°C Paenarthrobacter ureafaciens

Storage Stability

EC Number Storage Stability Organism
1.3.3.6 -20°C, potassium phosphate buffer, pH 7.0 Paenarthrobacter ureafaciens
1.3.3.6 37°C, 0.2 M potassium phosphate (pH 7.5) containing 0.1 M FAD, 4 h, less than 10% loss of activity Paenarthrobacter ureafaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.6 additional information high activity toward acyl-CoAs with a chain length of C12-C18, inactive with short chain acyl-CoAs with a chain length of C4 and C6 Paenarthrobacter ureafaciens ?
-
 ?
1.3.3.6 additional information exhibits high activity towards acyl-CoAs with chain lenghths of C12-C18 Paenarthrobacter ureafaciens ?
-
 ?
1.3.3.6 additional information high activity toward acyl-CoAs with a chain length of C12-C18, inactive with short chain acyl-CoAs with a chain length of C4 and C6 Paenarthrobacter ureafaciens NBRC 12140 ?
-
 ?
1.3.3.6 additional information exhibits high activity towards acyl-CoAs with chain lenghths of C12-C18 Paenarthrobacter ureafaciens NBRC 12140 ?
-
 ?
1.3.3.6 myristoyl-CoA + O2 highest activity Paenarthrobacter ureafaciens trans-2-tetradecenoyl-CoA + H2O2
-
 ir
1.3.3.6 myristoyl-CoA + O2 maximum activity Paenarthrobacter ureafaciens trans-2-tetradecenoyl-CoA + H2O2
-
 ?
1.3.3.6 myristoyl-CoA + O2 highest activity Paenarthrobacter ureafaciens NBRC 12140 trans-2-tetradecenoyl-CoA + H2O2
-
 ir
1.3.3.6 palmitoyl-CoA + O2
-
 Paenarthrobacter ureafaciens 2-trans-hexadecenoyl-CoA + H2O2
-
 ?
1.3.3.6 palmitoyl-CoA + O2
-
 Paenarthrobacter ureafaciens 2-trans-hexadecenoyl-CoA + H2O2
-
 ir
1.3.3.6 palmitoyl-CoA + O2
-
 Paenarthrobacter ureafaciens NBRC 12140 2-trans-hexadecenoyl-CoA + H2O2
-
 ?
1.3.3.6 palmitoyl-CoA + O2
-
 Paenarthrobacter ureafaciens NBRC 12140 2-trans-hexadecenoyl-CoA + H2O2
-
 ir

Subunits

EC Number Subunits Comment Organism
1.3.3.6 ? x * 76500, SDS-PAGE Paenarthrobacter ureafaciens
1.3.3.6 homodimer 2 * 75000, SDS-PAGE Paenarthrobacter ureafaciens
1.3.3.6 homodimer 2 * 95000, gel filtration Paenarthrobacter ureafaciens

Synonyms

EC Number Synonyms Comment Organism
1.3.3.6 ACO
-
 Candida sp. (in: Saccharomycetales)
1.3.3.6 ACO
-
 Paenarthrobacter ureafaciens
1.3.3.6 ACO
-
 Arthrobacter sp.
1.3.3.6 ACO-A1 recombinant enzyme Paenarthrobacter ureafaciens
1.3.3.6 acyl-coenzyme A oxidase
-
 Candida sp. (in: Saccharomycetales)
1.3.3.6 acyl-coenzyme A oxidase
-
 Paenarthrobacter ureafaciens
1.3.3.6 acyl-coenzyme A oxidase
-
 Arthrobacter sp.
1.3.3.6 acyl-coenzyme A: oxygen oxidoreductase
-
 Paenarthrobacter ureafaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.6 37
-
-
 Paenarthrobacter ureafaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.6 78
-
 palmitoyl-CoA
-
 Paenarthrobacter ureafaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.6 7.5
-
-
 Paenarthrobacter ureafaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.3.6 additional information
-
 between 6.5–7.5 at 37°C Paenarthrobacter ureafaciens
1.3.3.6 6.5 7.5 at 37°C Paenarthrobacter ureafaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.6 FAD
-
 Candida sp. (in: Saccharomycetales)
1.3.3.6 FAD
-
 Arthrobacter sp.
1.3.3.6 FAD one molecule of non-covalently bound FAD per subunit as a prosthetic group Paenarthrobacter ureafaciens
1.3.3.6 FAD flavoenzyme with 1 mol FAD per subunit Paenarthrobacter ureafaciens