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Literature summary extracted from

  • Li, H.; Melton, E.M.; Quackenbush, S.; DiRusso, C.C.; Black, P.N.
    Mechanistic studies of the long chain acyl-CoA synthetase Faa1p from Saccharomyces cerevisiae (2007), Biochim. Biophys. Acta, 1771, 1246-1253.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.3 expressed in Escherichia coli Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.3 0.0183
-
 CoA in 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, at 30°C Saccharomyces cerevisiae
6.2.1.3 0.0516
-
 ATP in 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, at 30°C Saccharomyces cerevisiae
6.2.1.3 0.0711
-
 oleate in 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, at 30°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.3 Mg2+ required, 10 mM Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.3 72500
-
 2 * 72500, gel filtration Saccharomyces cerevisiae
6.2.1.3 145000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.3 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.3 Ni-NTA resin column chromatography and Sephacryl S-200 HR gel filtration Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.3 0.121
-
 in the presence of 10 mM MnCl2, at pH 8.0 Saccharomyces cerevisiae
6.2.1.3 0.145
-
 in the presence of 10 mM MgCl2, at pH 8.0 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.3 ATP + oleate + CoA
-
 Saccharomyces cerevisiae AMP + diphosphate + oleoyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.3 homodimer 2 * 72500, gel filtration Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
6.2.1.3 ACSL
-
 Saccharomyces cerevisiae
6.2.1.3 Faa1p
-
 Saccharomyces cerevisiae
6.2.1.3 fatty acid CoA ligase: AMP forming
-
 Saccharomyces cerevisiae
6.2.1.3 long chain acyl-CoA synthetase
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.3 ATP
-
 Saccharomyces cerevisiae