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Literature summary extracted from

  • Hoffman, G.G.; Sona, S.; Bertin, M.; Ellington, W.R.
    The role of an absolutely conserved tryptophan residue in octamer formation and stability in mitochondrial creatine kinases (2006), Biochim. Biophys. Acta, 1764, 1512-1517.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.3.2 additional information the enzyme from Tethya aurantia lacks the absoluteley conserved Trp residue, Trp264 in chicken sarcomeric enzyme, involved in octamer stability in most organisms and consequently forms dimers. Mutation of Tyr residue in the site corresponding to the conserved Trp in other organisms to Trp, His or Asn also yields dimers Tethya aurantium
2.7.3.2 additional information the Trp residue corresponding to Trp264 in chicken sarcomeric enzyme is absoluteley conserved in most organisms and involved in octamer stability. Mutation of this Trp residue to Cys, Tyr, His, Asn, or Phe produces only dimers Chaetopterus variopedatus
2.7.3.2 W264C absoluteley conserved residue involved in octamer stability in most organisms, mutant forms dimers instead of octamers Gallus gallus
2.7.3.2 W264Y absoluteley conserved residue involved in octamer stability in most organisms, mutant forms dimers instead of octamers Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.3.2 mitochondrion
-
 Gallus gallus 5739
-
2.7.3.2 mitochondrion
-
 Chaetopterus variopedatus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Chaetopterus variopedatus
-
-
-
2.7.3.2 Gallus gallus
-
-
-
2.7.3.2 Tethya aurantium
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 sarcoma cell
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.3.2 22
-
 mutant Trp to Phe Chaetopterus variopedatus
2.7.3.2 26
-
 mutant Trp to Asn Chaetopterus variopedatus
2.7.3.2 26.8
-
 mutant Tyr to Asn Tethya aurantium
2.7.3.2 28.8
-
 mutant Tyr to His Tethya aurantium
2.7.3.2 29.6
-
 mutant Trp to Cys Chaetopterus variopedatus
2.7.3.2 31.8
-
 wild-type Tethya aurantium
2.7.3.2 32
-
 mutant Trp to His Chaetopterus variopedatus
2.7.3.2 36.2
-
 wild-type Chaetopterus variopedatus
2.7.3.2 36.4
-
 mutant Trp to Tyr Chaetopterus variopedatus
2.7.3.2 43.7
-
 mutant W264C Gallus gallus
2.7.3.2 62.3
-
 mutant W264Y Gallus gallus
2.7.3.2 70.2
-
 wild-type Gallus gallus
2.7.3.2 71.2
-
 mutant Tyr to Trp Tethya aurantium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ADP + phosphocreatine
-
 Gallus gallus ATP + creatine
-
 ?
2.7.3.2 ADP + phosphocreatine
-
 Tethya aurantium ATP + creatine
-
 ?
2.7.3.2 ADP + phosphocreatine
-
 Chaetopterus variopedatus ATP + creatine
-
 ?