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Literature summary extracted from

  • Ishmukhametov, R.R.; Galkin, M.A.; Vik, S.B.
    Ultrafast purification and reconstitution of His-tagged cysteine-less Escherichia coli F1Fo ATP synthase (2005), Biochim. Biophys. Acta, 1706, 110-116.
    View publication on PubMed

Application

EC Number Application Comment Organism
7.1.2.2 additional information incorporation of F1F0 into soybean liposomes yields well-coupled and highly active proteoliposomes Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.2.2 N,N'-dicyclohexylcarbodiimide 70% inhibition of the purified enzyme, 75% inhibition of the enzyme from mebrane vesicles Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.2.2 membrane
-
 Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.2.2 Mg2+ highest enzyme activity at 4-5 mM in the presence of 10 mM ATP Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
7.1.2.2 Escherichia coli
-
-
-
7.1.2.2 Escherichia coli DK8
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.2.2 His-tagged cysteine-less F1F0 ATP synthase, 80 fold by Ni-NTA affinity chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.2.2 ATP + H2O + H+/in
-
 Escherichia coli ADP + phosphate + H+/out
-
 r
7.1.2.2 ATP + H2O + H+/in
-
 Escherichia coli DK8 ADP + phosphate + H+/out
-
 r

Synonyms

EC Number Synonyms Comment Organism
7.1.2.2 F1F0 ATP synthase
-
 Escherichia coli