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Literature summary extracted from

  • Itoh, N.; Morinaga, N.; Kouzai, T.
    Purification and characterization of a novel metal-containing nonheme bromoperoxidase from Pseudomonas putida (1994), Biochim. Biophys. Acta, 1207, 208-216.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.B8 2-mercaptoethanol 0.1 mM, 97% inhibition Pseudomonas putida
1.11.1.B8 dithioerythritol
-
 Pseudomonas putida
1.11.1.B8 FeCl3 0.1 mM, 45% inhibition Pseudomonas putida
1.11.1.B8 hydrazine
-
 Pseudomonas putida
1.11.1.B8 hydroxylamine
-
 Pseudomonas putida
1.11.1.B8 KCN 0.1 mM, 82% inhibition Pseudomonas putida
1.11.1.B8 KF
-
 Pseudomonas putida
1.11.1.B8 monoiodoamine
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.B8 4.3
-
 H2O2
-
 Pseudomonas putida
1.11.1.B8 4.4
-
 Br-
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.B8 Co2+ contains 0.35 mol of cobalt ions per mol of enzyme. Addition of 0.1 mM CoCl2 results in 2.1fold increase in activity Pseudomonas putida
1.11.1.B8 Fe3+ contains 2.0 mol of ferric iron per mol of enzyme Pseudomonas putida
1.11.1.B8 additional information contains no vanadium ions Pseudomonas putida
1.11.1.B8 Ni2+ contains 1.0 mol of nickel ions per mol of enzyme Pseudomonas putida
1.11.1.B8 Zn2+ contains 0.8 mol of zinc ions per mol of enzyme Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.B8 33000
-
 2 * 33000 Pseudomonas putida
1.11.1.B8 68000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.B8 Pseudomonas putida
-
 IF-3 strain
-
1.11.1.B8 Pseudomonas putida IF-3
-
 IF-3 strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.B8
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.B8 9.38
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.B8 Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone Pseudomonas putida ?
-
 ?
1.11.1.B8 Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone Pseudomonas putida IF-3 ?
-
 ?
1.11.1.B8 I- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone Pseudomonas putida ?
-
 ?
1.11.1.B8 I- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone Pseudomonas putida IF-3 ?
-
 ?
1.11.1.B8 additional information inactive toward C1- and F- in the monochlorodimedone assay system Pseudomonas putida ?
-
 ?
1.11.1.B8 additional information inactive toward C1- and F- in the monochlorodimedone assay system Pseudomonas putida IF-3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.B8 dimer 2 * 33000 Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.11.1.B8 nonheme bromoperoxidase
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.B8
-
 20 pH 7.0, 20 min, stable Pseudomonas putida
1.11.1.B8 30 35 pH 7.0, 20 min, 10% loss of activity Pseudomonas putida
1.11.1.B8 40
-
 pH 7.0, 20 min, 20% loss of activity Pseudomonas putida
1.11.1.B8 50 60 pH 7.0, 20 min, 10% loss of activity Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.B8 4.2
-
-
 Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.B8 3.4
-
 2 h, 25°C, complete loss of activity below pH 3.5 Pseudomonas putida
1.11.1.B8 4 11 2 h, 25°C, stable Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.B8 additional information contains no heme Pseudomonas putida