Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhang, J.W.; Guo, Q.; Zhao, T.J.; Liu, T.T.; Wang, X.C.
    Two fused proteins combining Stichopus japonicus arginine kinase and rabbit muscle creatine kinase (2006), Biochemistry (Moscow), 71, 983-988.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.3 expressed in Escherichia coli strain BL21 (DE3) Apostichopus japonicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.3.2 additional information fusion proteins of Stichopus japonicus arginine kinase and rabbit muscle creatine kinase in direction arginine kinase-creatine kinase, AK-CK and creatine kinase-arginine kinase, CK-AK. In both fusion proteins, both of the enzymes show about 50% decrease in activity and about 2fold Km values. Fused proteins have similar secondary structure, tertiary structure, molecular size, and thermodynamic stability Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.3.3 guanidine hydrochloride 0.2 mM, about 90% loss of activity Apostichopus japonicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.2 0.4
-
 ATP wild-type Oryctolagus cuniculus
2.7.3.2 0.73
-
 ATP fusion protein CK-AK Oryctolagus cuniculus
2.7.3.2 0.86
-
 ATP fusion protein AK-CK Oryctolagus cuniculus
2.7.3.2 6.4
-
 Creatine wild-type Oryctolagus cuniculus
2.7.3.2 13
-
 Creatine fusion protein CK-AK Oryctolagus cuniculus
2.7.3.2 14
-
 Creatine fusion protein AK-CK Oryctolagus cuniculus
2.7.3.3 0.52
-
 L-Arg at 25°C Apostichopus japonicus
2.7.3.3 1.01
-
 ATP at 25°C Apostichopus japonicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.3 Mg2+ required in complex with ATP Apostichopus japonicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.3 42000
-
 2 * 42000, SDS-PAGE Apostichopus japonicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Oryctolagus cuniculus
-
-
-
2.7.3.3 Apostichopus japonicus
-
 sea cucumber
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.3 Ni2+-NTA column chromatography and Superdex 200HR 10/30 gel filtration Apostichopus japonicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ATP + creatine
-
 Oryctolagus cuniculus ADP + creatine phosphate
-
 ?
2.7.3.3 ATP + L-Arg
-
 Apostichopus japonicus ADP + Nomega-phospho-L-Arg
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.3.3 dimer 2 * 42000, SDS-PAGE Apostichopus japonicus

Synonyms

EC Number Synonyms Comment Organism
2.7.3.3 ATP:arginine N-phosphotransferase
-
 Apostichopus japonicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.3.3 ATP
-
 Apostichopus japonicus