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Literature summary extracted from

  • Shleev, S.V.; Shumakovich, G.P.; Nikitina, O.V.; Morozova, O.V.; Pavlishko, H.M.; Gayda, G.Z.; Gonchar, M.V.
    Purification and characterization of alcohol oxidase from a genetically constructed over-producing strain of the methylotrophic yeast Hansenula polymorpha (2006), Biochemistry (Moscow), 71, 245-250.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.13 NaN3 competitive, reversible Ogataea angusta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.13 0.4
-
 methanol pH 7.5, 30°C Ogataea angusta
1.1.3.13 6.6
-
 ethanol pH 7.5, 30°C Ogataea angusta
1.1.3.13 10.5
-
 formaldehyde pH 7.5, 30°C Ogataea angusta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.13 80000
-
 8 * 80000, SDS-PAGE Ogataea angusta
1.1.3.13 620000
-
 gel filtration Ogataea angusta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.13 ethanol + O2 Ogataea angusta
-
 acetaldehyde + H2O2
-
 ?
1.1.3.13 formaldehyde + O2 Ogataea angusta
-
 formic acid + H2O2
-
 ?
1.1.3.13 methanol + O2 Ogataea angusta
-
 formaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Ogataea angusta
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13 recombinant enzyme 7.7fold from overproducing Hansenula polymorpha strain C-105 by ammonium sulfate fractionation and ion exchange chromatography to homogeneity Ogataea angusta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.13 23
-
 purified enzyme Ogataea angusta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 ethanol + O2
-
 Ogataea angusta acetaldehyde + H2O2
-
 ?
1.1.3.13 formaldehyde + O2
-
 Ogataea angusta formic acid + H2O2
-
 ?
1.1.3.13 formaldehyde + O2 low activity Ogataea angusta formic acid + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 Ogataea angusta formaldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.13 More secondary structure analysis, overview Ogataea angusta
1.1.3.13 octamer 8 * 80000, SDS-PAGE Ogataea angusta

Synonyms

EC Number Synonyms Comment Organism
1.1.3.13 AOX
-
 Ogataea angusta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.13 30
-
 assay at Ogataea angusta

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.13 32
-
 formaldehyde pH 7.5, 30°C Ogataea angusta
1.1.3.13 58
-
 ethanol pH 7.5, 30°C Ogataea angusta
1.1.3.13 60
-
 methanol pH 7.5, 30°C Ogataea angusta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.13 7.5
-
 assay at Ogataea angusta

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.13 FAD
-
 Ogataea angusta

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.13 Ogataea angusta isoelectric focusing
-
 6.1