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Literature summary extracted from

  • Morozkina, E.V.; Nosikov, A.N.; Zvyagilskaya, R.A.; Lvov, N.P.
    Isolation, purification, and characterization of nitrate reductase from a salt-tolerant Rhodotorula glutinis yeast strain grown in the presence of tungsten (2005), Biochemistry (Moscow), 70, 809-814.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.2 azide at low concentration Rhodotorula glutinis
1.7.1.2 cyanide at low concentration Rhodotorula glutinis
1.7.1.2 additional information insensitive to 1 mM tungsten Rhodotorula glutinis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.2 0.74
-
 nitrate apparent KM Rhodotorula glutinis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.2 Molybdenum contains 0.177 mol of Mo per mol Rhodotorula glutinis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.2 65000
-
 2 * 65000, SDS-PAGE Rhodotorula glutinis
1.7.1.2 130000
-
 SDS-PAGE Rhodotorula glutinis

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.2 Rhodotorula glutinis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.2 DEAE-Toyopearl 650M column chromatography, Sephacryl S-200 gel filtration and DEAE-cellulose DE-52 column chromatography Rhodotorula glutinis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.2 1.3
-
 after purification Rhodotorula glutinis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.2 nitrate + NAD(P)H
-
 Rhodotorula glutinis nitrite + NAD(P)+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.2 homodimer 2 * 65000, SDS-PAGE Rhodotorula glutinis

Synonyms

EC Number Synonyms Comment Organism
1.7.1.2 NAD(P)H:nitrate-oxidoreductase
-
 Rhodotorula glutinis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.2 35 45
-
 Rhodotorula glutinis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.2 7
-
-
 Rhodotorula glutinis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.1.2 6.5 7.5
-
 Rhodotorula glutinis

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.2 NAD(P)H
-
 Rhodotorula glutinis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.1.2 0.0094
-
 cyanide at low concentration Rhodotorula glutinis
1.7.1.2 0.044
-
 azide at low concentration Rhodotorula glutinis