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Literature summary extracted from

  • Panova, N.G.; Alexeev, C.S.; Kuzmichov, A.S.; Shcheveleva, E.V.; Gavryushov, S.A.; Polyakov, K.M.; Kritzyn, A.M.; Mikhailov, S.N.; Esipov, R.S.; Miroshnikov, A.I.
    Substrate specificity of Escherichia coli thymidine phosphorylase (2007), Biochemistry, 72, 21-28.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.4 1-(2''-deoxy-beta-D-threo-pentafuranosyl)thymine
-
 Escherichia coli
2.4.2.4 1-(2'-deoxy-beta-D-threo-pentafuranosyl)thymine
-
 Escherichia coli
2.4.2.4 3'-amino-3'-deoxythymidine
-
 Escherichia coli
2.4.2.4 3'-Deoxythymidine
-
 Escherichia coli
2.4.2.4 5'-azido-5'-deoxythymidine
-
 Escherichia coli
2.4.2.4 5'-bromo-5'-deoxythymidine
-
 Escherichia coli
2.4.2.4 5'-chloro-5'-deoxythymidine
-
 Escherichia coli
2.4.2.4 5'-iodo-5'-deoxythymidine
-
 Escherichia coli
2.4.2.4 ftorafur ftorafur is also called tegafur Escherichia coli
2.4.2.4 methyluridine
-
 Escherichia coli
2.4.2.4 uridine
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.4 0.28
-
 4-thiothymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.28
-
 4-thiothymidine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 0.3
-
 thymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.3
-
 1-(2',6'-dideoxy-beta-D-ribohexafuranosyl)thymine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.3
-
 5'-chloro-5'-deoxythymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.3
-
 thymidine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 0.3
-
 1-(2',6'-dideoxy-beta-D-ribohexafuranosyl)thymine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 0.32
-
 2'-deoxyuridine
-
 Escherichia coli
2.4.2.4 0.33
-
 1-(2',6'-dideoxy-beta-D-ribo-hexafuranosyl)thymine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.33
-
 1-(2',6'-dideoxy-beta-D-ribohexafuranosyl)thymine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 0.35
-
 3'-deoxy-2',3'-didehydrothymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.35
-
 1-(2',6'-dideoxy-alpha-L-lyxo-hexafuranosyl)thymine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.35
-
 3'-deoxy-2',3'-didehydrothymidine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 0.35
-
 1-(2',6'-dideoxy-alpha-L-lyxohexafuranosyl)thymine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 0.4
-
 5'-Deoxythymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.4
-
 3'-amino-3'-deoxythymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.4
-
 5'-amino-5'-deoxythymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.4
-
 5'-Deoxythymidine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 0.4
-
 3'-amino-3'-deoxythymidine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 0.4
-
 5'-amino-5'-deoxythymidine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.4 Escherichia coli
-
-
-
2.4.2.4 Escherichia coli P07650
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.4 1-(2',6'-dideoxy-alpha-L-lyxo-hexafuranosyl)thymine + phosphate
-
 Escherichia coli ?
-
 ?
2.4.2.4 1-(2',6'-dideoxy-alpha-L-lyxohexafuranosyl)thymine + phosphate
-
 Escherichia coli thymine + 2,6-dideoxy-L-lyxohexafuranose 1-phosphate
-
 ?
2.4.2.4 1-(2',6'-dideoxy-beta-D-ribo-hexafuranosyl)thymine + phosphate
-
 Escherichia coli ?
-
 ?
2.4.2.4 1-(2',6'-dideoxy-beta-D-ribohexafuranosyl)thymine + phosphate
-
 Escherichia coli ?
-
 ?
2.4.2.4 1-(2',6'-dideoxy-beta-D-ribohexafuranosyl)thymine + phosphate
-
 Escherichia coli thymine + 2,6-dideoxy-D-ribohexafuranose 1-phosphate
-
 ?
2.4.2.4 2'-deoxyuridine + phosphate
-
 Escherichia coli ?
-
 ?
2.4.2.4 3'-amino-3'-deoxythymidine + phosphate
-
 Escherichia coli ?
-
 ?
2.4.2.4 3'-amino-3'-deoxythymidine + phosphate at pH 8.0 Escherichia coli ?
-
 ?
2.4.2.4 3'-deoxy-2',3'-didehydrothymidine + phosphate
-
 Escherichia coli ?
-
 ?
2.4.2.4 3'-deoxy-2',3'-didehydrothymidine + phosphate
-
 Escherichia coli thymine + 2,3-dideoxy-2,3-didehydro-D-ribose 1-phosphate
-
 ?
2.4.2.4 4-thiothymidine + phosphate
-
 Escherichia coli 4-thiothymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.4 4-thiothymidine + phosphate
-
 Escherichia coli ?
-
 ?
2.4.2.4 5'-amino-5'-deoxythymidine + phosphate
-
 Escherichia coli ?
-
 ?
2.4.2.4 5'-amino-5'-deoxythymidine + phosphate
-
 Escherichia coli thymine + 2,5-dideoxy-5-amino-D-ribose 1-phosphate
-
 ?
2.4.2.4 5'-chloro-5'-deoxythymidine + phosphate
-
 Escherichia coli ?
-
 ?
2.4.2.4 5'-deoxythymidine + phosphate
-
 Escherichia coli ?
-
 ?
2.4.2.4 5'-deoxythymidine + phosphate
-
 Escherichia coli thymine + 2,5-dideoxy-D-ribose 1-phosphate
-
 ?
2.4.2.4 additional information 3'-amino-3'-deoxythymidine is not a thymidine phosphorylase substrate at pH 6.5, no activity with uridine and methyluridine Escherichia coli ?
-
 ?
2.4.2.4 thymidine + phosphate
-
 Escherichia coli thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.4 0.1
-
 1-(2',6'-dideoxy-beta-D-ribo-hexafuranosyl)thymine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.1
-
 1-(2',6'-dideoxy-beta-D-ribohexafuranosyl)thymine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 0.4
-
 3'-deoxy-2',3'-didehydrothymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.4
-
 3'-deoxy-2',3'-didehydrothymidine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 0.45
-
 1-(2',6'-dideoxy-alpha-L-lyxo-hexafuranosyl)thymine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.45
-
 1-(2',6'-dideoxy-alpha-L-lyxohexafuranosyl)thymine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 0.58
-
 5'-amino-5'-deoxythymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.58
-
 5'-amino-5'-deoxythymidine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 0.6
-
 1-(2',6'-dideoxy-beta-D-ribohexafuranosyl)thymine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.6
-
 1-(2',6'-dideoxy-beta-D-ribohexafuranosyl)thymine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 1.1
-
 3'-amino-3'-deoxythymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 1.1
-
 3'-amino-3'-deoxythymidine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 1.7
-
 5'-chloro-5'-deoxythymidine
-
 Escherichia coli
2.4.2.4 35
-
 2'-deoxyuridine
-
 Escherichia coli
2.4.2.4 63
-
 4-thiothymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 63
-
 4-thiothymidine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 198
-
 thymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 198
-
 thymidine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli
2.4.2.4 260
-
 5'-Deoxythymidine
-
 Escherichia coli
2.4.2.4 260
-
 5'-Deoxythymidine in 50 mM potassium phosphate buffer (pH 6.5), at 25°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.4 6.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.4 6.1 6.8
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.4 0.06
-
 uridine in 50 mM potassium phosphate buffer, pH 6.5, 25°C Escherichia coli
2.4.2.4 0.12
-
 methyluridine in 50 mM potassium phosphate buffer, pH 6.5, 25°C Escherichia coli
2.4.2.4 0.4
-
 5'-bromo-5'-deoxythymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.4
-
 5'-iodo-5'-deoxythymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.4
-
 5'-bromo-5'-deoxythymidine in 50 mM potassium phosphate buffer, pH 6.5, 25°C Escherichia coli
2.4.2.4 0.4
-
 5'-iodo-5'-deoxythymidine in 50 mM potassium phosphate buffer, pH 6.5, 25°C Escherichia coli
2.4.2.4 0.45
-
 ftorafur 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.45
-
 1-(2''-deoxy-beta-D-threo-pentafuranosyl)thymine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.45
-
 ftorafur in 50 mM potassium phosphate buffer, pH 6.5, 25°C Escherichia coli
2.4.2.4 0.45
-
 1-(2'-deoxy-beta-D-threo-pentafuranosyl)thymine in 50 mM potassium phosphate buffer, pH 6.5, 25°C Escherichia coli
2.4.2.4 0.6
-
 3'-amino-3'-deoxythymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.6
-
 3'-amino-3'-deoxythymidine in 50 mM potassium phosphate buffer, pH 6.5, 25°C Escherichia coli
2.4.2.4 0.85
-
 3'-Deoxythymidine 25°C, pH 6.5 Escherichia coli
2.4.2.4 0.85
-
 3'-Deoxythymidine in 50 mM potassium phosphate buffer, pH 6.5, 25°C Escherichia coli