BRENDA - Enzyme Database

Regulation of 2-oxoglutarate metabolism in rat liver by NADP-isocitrate dehydrogenase and aspartate aminotransferase

Rakhmanova, T.I.; Popova, T.N.; Biochemistry 71, 211-217 (2006)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.6.1.1
aconitate
activating effect on the activity of the cytoplasmic enzyme form
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.1
fumarate
-
Rattus norvegicus
2.6.1.1
isocitrate
-
Rattus norvegicus
2.6.1.1
L-aspartate
-
Rattus norvegicus
2.6.1.1
L-glutamate
-
Rattus norvegicus
2.6.1.1
L-glutamine
-
Rattus norvegicus
2.6.1.1
succinate
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.1
0.35
-
L-aspartate
cytoplasmic enzyme
Rattus norvegicus
2.6.1.1
0.75
-
L-aspartate
mitochondrial enzyme
Rattus norvegicus
2.6.1.1
1.25
-
2-oxoglutarate
cytoplasmic enzyme
Rattus norvegicus
2.6.1.1
1.75
-
2-oxoglutarate
mitochondrial enzyme
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.1
cytoplasm
-
Rattus norvegicus
5737
-
2.6.1.1
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.1
92000
-
mitochondrial form, SDS-PAGE
Rattus norvegicus
2.6.1.1
98000
-
cytoplasmic form, SDS-PAGE
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.1
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.6.1.1
differential centrifugation, Sephadex G-25 gel filtration, DEAE cellulose ion exchange chromatography, and Sephadex G-150 gel filtration
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.1
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.1
22.37
-
mitochondrial enzyme
Rattus norvegicus
2.6.1.1
48.45
-
cytoplasmic enzyme
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.1
L-aspartate + 2-oxoglutarate
-
672283
Rattus norvegicus
oxaloacetate + L-glutamate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.1
6.8
-
mitochondrial form
Rattus norvegicus
2.6.1.1
7.5
-
cytoplasmic form
Rattus norvegicus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.6.1.1
1.6
-
L-aspartate
cytoplasmic enzyme
Rattus norvegicus
2.6.1.1
1.8
-
L-glutamate
mitochondrial enzyme
Rattus norvegicus
2.6.1.1
1.9
-
L-aspartate
mitochondrial enzyme
Rattus norvegicus
2.6.1.1
2.4
-
L-glutamate
cytoplasmic enzyme
Rattus norvegicus
2.6.1.1
2.6
-
L-glutamine
mitochondrial enzyme
Rattus norvegicus
2.6.1.1
3.7
-
L-glutamine
cytoplasmic enzyme
Rattus norvegicus
2.6.1.1
4.1
-
succinate
mitochondrial enzyme
Rattus norvegicus
2.6.1.1
5.7
-
succinate
cytoplasmic enzyme
Rattus norvegicus
2.6.1.1
6.6
-
isocitrate
cytoplasmic enzyme
Rattus norvegicus
2.6.1.1
8.4
-
fumarate
cytoplasmic enzyme
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.6.1.1
aconitate
activating effect on the activity of the cytoplasmic enzyme form
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.6.1.1
fumarate
-
Rattus norvegicus
2.6.1.1
isocitrate
-
Rattus norvegicus
2.6.1.1
L-aspartate
-
Rattus norvegicus
2.6.1.1
L-glutamate
-
Rattus norvegicus
2.6.1.1
L-glutamine
-
Rattus norvegicus
2.6.1.1
succinate
-
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.6.1.1
1.6
-
L-aspartate
cytoplasmic enzyme
Rattus norvegicus
2.6.1.1
1.8
-
L-glutamate
mitochondrial enzyme
Rattus norvegicus
2.6.1.1
1.9
-
L-aspartate
mitochondrial enzyme
Rattus norvegicus
2.6.1.1
2.4
-
L-glutamate
cytoplasmic enzyme
Rattus norvegicus
2.6.1.1
2.6
-
L-glutamine
mitochondrial enzyme
Rattus norvegicus
2.6.1.1
3.7
-
L-glutamine
cytoplasmic enzyme
Rattus norvegicus
2.6.1.1
4.1
-
succinate
mitochondrial enzyme
Rattus norvegicus
2.6.1.1
5.7
-
succinate
cytoplasmic enzyme
Rattus norvegicus
2.6.1.1
6.6
-
isocitrate
cytoplasmic enzyme
Rattus norvegicus
2.6.1.1
8.4
-
fumarate
cytoplasmic enzyme
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6.1.1
0.35
-
L-aspartate
cytoplasmic enzyme
Rattus norvegicus
2.6.1.1
0.75
-
L-aspartate
mitochondrial enzyme
Rattus norvegicus
2.6.1.1
1.25
-
2-oxoglutarate
cytoplasmic enzyme
Rattus norvegicus
2.6.1.1
1.75
-
2-oxoglutarate
mitochondrial enzyme
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.6.1.1
cytoplasm
-
Rattus norvegicus
5737
-
2.6.1.1
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.1
92000
-
mitochondrial form, SDS-PAGE
Rattus norvegicus
2.6.1.1
98000
-
cytoplasmic form, SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.1
differential centrifugation, Sephadex G-25 gel filtration, DEAE cellulose ion exchange chromatography, and Sephadex G-150 gel filtration
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.6.1.1
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6.1.1
22.37
-
mitochondrial enzyme
Rattus norvegicus
2.6.1.1
48.45
-
cytoplasmic enzyme
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.1
L-aspartate + 2-oxoglutarate
-
672283
Rattus norvegicus
oxaloacetate + L-glutamate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.1
6.8
-
mitochondrial form
Rattus norvegicus
2.6.1.1
7.5
-
cytoplasmic form
Rattus norvegicus