BRENDA - Enzyme Database

Modulation of diacylglycerol kinase theta activity by alpha-thrombin and phospholipids

Tu-Sekine, B.; Ostroski, M.; Raben, D.M.; Biochemistry 46, 924-932 (2007)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.107
phosphatidic acid
more effective activator than phosphatidylserine. Phosphatidic acid decreases the apparent surface KM of DGKtheta for dioleoylglycerol and promotes binding to vesicles in a dose-dependent manner; phosphatidic acid is more effective than phosphatidiylserine. Both decreases the apparent surface KM value for dioleoylglycerol and promote binding to vesicles, but through different mechanisms
Homo sapiens
2.7.1.107
phosphatidylserine
less effective activator than phosphatidic acid. Phosphatidylserine decreases the apparent surface KM of DGKtheta for dioleoylglycerol; phosphatidic acid is more effective than phosphatidiylserine. Both decreases the apparent surface KM value for dioleoylglycerol and promote binding to vesicles, but through different mechanisms
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.107
expression in IIC9 cells
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.107
alpha-thrombin
apparent binding parameters of diacylglycerol kinase theta increase following alpha-thrombin stimulation
Homo sapiens
2.7.1.107
thrombin
stimulation results in an increase in the apparent KM of DGKtheta at low concentrations of substrate dioleoylglycerol. Increasing the bulk concentration of dioleoylglycerol returns the apparent KM to the basal value
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.107
additional information
-
additional information
KMapp for substrate 1,2-dioleoyl-sn-glycerol is 0.9 mol% in presence of 1% phosphatidic acid, 0.6 mol% in presence of 9% phosphatidylserine, and 8.0 mol% without additive
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.107
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.107
ATP + 1,2-dioleoyl-sn-glycerol
-
672275
Homo sapiens
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.107
phosphatidic acid
more effective activator than phosphatidylserine. Phosphatidic acid decreases the apparent surface KM of DGKtheta for dioleoylglycerol and promotes binding to vesicles in a dose-dependent manner; phosphatidic acid is more effective than phosphatidiylserine. Both decreases the apparent surface KM value for dioleoylglycerol and promote binding to vesicles, but through different mechanisms
Homo sapiens
2.7.1.107
phosphatidylserine
less effective activator than phosphatidic acid. Phosphatidylserine decreases the apparent surface KM of DGKtheta for dioleoylglycerol; phosphatidic acid is more effective than phosphatidiylserine. Both decreases the apparent surface KM value for dioleoylglycerol and promote binding to vesicles, but through different mechanisms
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.107
expression in IIC9 cells
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.107
alpha-thrombin
apparent binding parameters of diacylglycerol kinase theta increase following alpha-thrombin stimulation
Homo sapiens
2.7.1.107
thrombin
stimulation results in an increase in the apparent KM of DGKtheta at low concentrations of substrate dioleoylglycerol. Increasing the bulk concentration of dioleoylglycerol returns the apparent KM to the basal value
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.107
additional information
-
additional information
KMapp for substrate 1,2-dioleoyl-sn-glycerol is 0.9 mol% in presence of 1% phosphatidic acid, 0.6 mol% in presence of 9% phosphatidylserine, and 8.0 mol% without additive
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.107
ATP + 1,2-dioleoyl-sn-glycerol
-
672275
Homo sapiens
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
-
-
?