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Literature summary extracted from

  • Tu-Sekine, B.; Ostroski, M.; Raben, D.M.
    Modulation of diacylglycerol kinase theta activity by alpha-thrombin and phospholipids (2007), Biochemistry, 46, 924-932.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.107 phosphatidic acid more effective activator than phosphatidylserine. Phosphatidic acid decreases the apparent surface KM of DGKtheta for dioleoylglycerol and promotes binding to vesicles in a dose-dependent manner Homo sapiens
2.7.1.107 phosphatidic acid phosphatidic acid is more effective than phosphatidiylserine. Both decreases the apparent surface KM value for dioleoylglycerol and promote binding to vesicles, but through different mechanisms Homo sapiens
2.7.1.107 phosphatidylserine less effective activator than phosphatidic acid. Phosphatidylserine decreases the apparent surface KM of DGKtheta for dioleoylglycerol Homo sapiens
2.7.1.107 phosphatidylserine phosphatidic acid is more effective than phosphatidiylserine. Both decreases the apparent surface KM value for dioleoylglycerol and promote binding to vesicles, but through different mechanisms Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.107 expression in IIC9 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.107 alpha-thrombin apparent binding parameters of diacylglycerol kinase theta increase following alpha-thrombin stimulation Homo sapiens
2.7.1.107 thrombin stimulation results in an increase in the apparent KM of DGKtheta at low concentrations of substrate dioleoylglycerol. Increasing the bulk concentration of dioleoylglycerol returns the apparent KM to the basal value Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 additional information
-
 additional information KMapp for substrate 1,2-dioleoyl-sn-glycerol is 0.9 mol% in presence of 1% phosphatidic acid, 0.6 mol% in presence of 9% phosphatidylserine, and 8.0 mol% without additive Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-dioleoyl-sn-glycerol
-
 Homo sapiens ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
 ?