EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.1.13 | expression of His-tagged enzyme in Escherichia coli strain BL21 | Mycolicibacterium smegmatis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.13 | 5'-O-[N-(L-cysteinyl)sulfamonyl]adenosine | a stable bisubstrate analogue, exhibits competitive inhibition versus ATP and noncompetitive inhibition versus cysteine | Mycolicibacterium smegmatis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.13 | additional information | - |
additional information | steady-state and pre-steady-state kinetic analysis, single-turnover reactions of the first and second half reactions | Mycolicibacterium smegmatis | |
6.3.1.13 | 0.1 | - |
L-cysteine | pH 7.8, 25°C, recombinant enzyme | Mycolicibacterium smegmatis | |
6.3.1.13 | 0.16 | - |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol | pH 7.8, 25°C, recombinant enzyme | Mycolicibacterium smegmatis | |
6.3.1.13 | 1.8 | - |
ATP | pH 7.8, 25°C, recombinant enzyme | Mycolicibacterium smegmatis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.13 | Mg2+ | - |
Mycolicibacterium smegmatis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.1.13 | 40700 | - |
recombinant His-tagged enzyme, gel filtration | Mycolicibacterium smegmatis |
6.3.1.13 | 47000 | - |
1 * 47000, recombinant His-tagged enzyme, 1 * 47562, amino acid sequence calculation | Mycolicibacterium smegmatis |
6.3.1.13 | 47562 | - |
1 * 47000, recombinant His-tagged enzyme, 1 * 47562, amino acid sequence calculation | Mycolicibacterium smegmatis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.13 | 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | Mycolicibacterium smegmatis | a key step in mycothiol, i.e. 1-D-myo-inosityl-2-(N-acetyl-L-cysteinyl)amido-2-deoxy-R-D-glucopyranoside or MSH or AcCys-GlcN-Ins, biosynthesis, mycothiol is produced to act against oxidative and antibiotic stress and is essential for cell growth, biosynthetic pathway, overview | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.1.13 | Mycolicibacterium smegmatis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.1.13 | recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration to about 95% purity | Mycolicibacterium smegmatis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.3.1.13 | 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | Bi Uni Uni Bi Ping Pong reaction mechanism | Mycolicibacterium smegmatis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.13 | 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | - |
Mycolicibacterium smegmatis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
6.3.1.13 | 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | a key step in mycothiol, i.e. 1-D-myo-inosityl-2-(N-acetyl-L-cysteinyl)amido-2-deoxy-R-D-glucopyranoside or MSH or AcCys-GlcN-Ins, biosynthesis, mycothiol is produced to act against oxidative and antibiotic stress and is essential for cell growth, biosynthetic pathway, overview | Mycolicibacterium smegmatis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.1.13 | monomer | 1 * 47000, recombinant His-tagged enzyme, 1 * 47562, amino acid sequence calculation | Mycolicibacterium smegmatis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.1.13 | cysteine ligase | - |
Mycolicibacterium smegmatis |
6.3.1.13 | MshC | - |
Mycolicibacterium smegmatis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.1.13 | 25 | - |
assay at | Mycolicibacterium smegmatis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.13 | 3.15 | - |
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol | pH 7.8, 25°C, recombinant enzyme | Mycolicibacterium smegmatis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.1.13 | 7.8 | - |
assay at | Mycolicibacterium smegmatis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.13 | ATP | - |
Mycolicibacterium smegmatis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.13 | 0.00031 | - |
5'-O-[N-(L-cysteinyl)sulfamonyl]adenosine | versus ATP | Mycolicibacterium smegmatis |