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Literature summary extracted from

  • Levengood, J.D.; Roy, H.; Ishitani, R.; Soell, D.; Nureki, O.; Ibba, M.
    Anticodon recognition and discrimination by the alpha-helix cage domain of class I lysyl-tRNA synthetase (2007), Biochemistry, 46, 11033-11038.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.6 expressed in Escherichia coli BL21(DE3+ RPC) cells Pyrococcus horikoshii

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.6 Y491E exhibits significant improvement compared to the wild type in aminoacylation of a tRNALysUUG Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.6 0.87
-
 tRNALysUUU wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP Pyrococcus horikoshii
6.1.1.6 1.4
-
 tRNALysCUU wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP Pyrococcus horikoshii
6.1.1.6 1.5
-
 tRNALysGUU wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.6 Pyrococcus horikoshii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.6 MonoQ 5/5 column chromatography and Superose 12 gel filtration Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.6 ATP + L-lysine + tRNALysCUU
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALysCUU + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALysGUU
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALysGUU + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALysUCU
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALysUCU + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALysUGU
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALysUGU + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALysUUC
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALysUUC + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALysUUG
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALysUUG + diphosphate
-
 ?
6.1.1.6 ATP + L-lysine + tRNALysUUU
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALysUUU + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.6 class I lysyl-tRNA synthetase
-
 Pyrococcus horikoshii
6.1.1.6 LysRS1
-
 Pyrococcus horikoshii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.6 0.043
-
 tRNALysGUU wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP Pyrococcus horikoshii
6.1.1.6 0.062
-
 tRNALysCUU wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP Pyrococcus horikoshii
6.1.1.6 0.44
-
 tRNALysUUU wild type enzyme, at 37°C in 100 mM HEPES (pH 7.2), 25 mM KCl, 10 mM MgCl2, 4 mM dithiothreitol, and 5 mM ATP Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.6 ATP
-
 Pyrococcus horikoshii