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Literature summary extracted from

  • Pinto, A.; Tarasev, M.; Ballou, D.P.
    Substitutions of the bridging aspartate 178 result in profound changes in the reactivity of the Rieske center of phthalate dioxygenase (2006), Biochemistry, 45, 9032-9041.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.7 expressed in Escherichia coli strain C41(DE3) Burkholderia cepacia

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.7 D178A the rates of oxidation of the reduced Rieske centers are decreased by more than 10000fold, 16% of wild type activity Burkholderia cepacia
1.14.12.7 D178N the rates of oxidation of the reduced Rieske centers are decreased by more than 10000fold, 7% of wild type activity Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.7 0.0002
-
 Phthalate wild type enzyme with its mononuclear center containing Fe(II) Burkholderia cepacia
1.14.12.7 0.0009
-
 Phthalate mutant enzyme D178A with its mononuclear center lacking Fe(II) Burkholderia cepacia
1.14.12.7 0.0011
-
 Phthalate mutant enzyme D178N with its mononuclear center lacking Fe(II) Burkholderia cepacia
1.14.12.7 0.0016
-
 Phthalate wild type enzyme with its mononuclear center lacking Fe(II) Burkholderia cepacia
1.14.12.7 0.0058
-
 Phthalate mutant enzyme D178N with its mononuclear center containing Fe(II) Burkholderia cepacia
1.14.12.7 0.0093
-
 Phthalate mutant enzyme D178A with its mononuclear center containing Fe(II) Burkholderia cepacia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.7 Fe2+ the mononuclear center contains Fe(II), contains 2.85 Fe atoms per monomer Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.7 Burkholderia cepacia
-
-
-
1.14.12.7 Burkholderia cepacia DB01
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.7
-
 Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.7 phthalate + NADH + O2
-
 Burkholderia cepacia cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O
-
 ?
1.14.12.7 phthalate + NADH + O2
-
 Burkholderia cepacia DB01 cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.7 PDO
-
 Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.7 NADH
-
 Burkholderia cepacia