EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.214 | expression in Escherichia coli DH5alpha or BL21 | Helicobacter pylori |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.214 | additional information | structural analysis of the FT of Helicobacter pylori by systematic C-terminal truncation reveals that up to 80 residues, including the region rich in hydrophobic and positively charged residues (434-478) and 5 of the 10 tandem repeats of 7 amino acids each (399-433) can be removed without significant change in structure and catalysis, half of the heptad repeats required to maintain both the secondary and native quaternary structures, systematic deletion of the C-terminus considerably improves the solubility of the protein, truncated forms are highly specific for type 2 sugar substrates that contain the Galbeta-1,4-GlcNAc structure, type 1 substrate Galbeta-1,3-GlcNAc is not accepted | Helicobacter pylori |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.214 | 0.0683 | - |
GDP-fucose | deletion mutant FTdelta23 with 23 residues deleted from the C-terminus | Helicobacter pylori | |
2.4.1.214 | 0.07864 | - |
GDP-fucose | deletion mutant FTdelta66 with 66 residues deleted from the C-terminus | Helicobacter pylori | |
2.4.1.214 | 0.07967 | - |
GDP-fucose | deletion mutant FTdelta38 with 38 residues deleted from the C-terminus | Helicobacter pylori | |
2.4.1.214 | 0.08831 | - |
GDP-fucose | deletion mutant FTdelta45 with 45 residues deleted from the C-terminus | Helicobacter pylori | |
2.4.1.214 | 0.09356 | - |
GDP-fucose | deletion mutant FTdelta28 with 28 residues deleted from the C-terminus | Helicobacter pylori | |
2.4.1.214 | 0.09864 | - |
GDP-fucose | deletion mutant FTdelta80 with 80 residues deleted from the C-terminus | Helicobacter pylori | |
2.4.1.214 | 0.228 | - |
GDP-fucose | deletion mutant FTdelta115 with 115 residues deleted from the C-terminus | Helicobacter pylori | |
2.4.1.214 | 0.7 | - |
LacNAc | deletion mutant FTdelta38 with 38 residues deleted from the C-terminus | Helicobacter pylori | |
2.4.1.214 | 0.71 | - |
LacNAc | deletion mutant FTdelta45 with 45 residues deleted from the C-terminus | Helicobacter pylori | |
2.4.1.214 | 0.71 | - |
LacNAc | deletion mutant FTdelta66 with 66 residues deleted from the C-terminus | Helicobacter pylori | |
2.4.1.214 | 0.78 | - |
LacNAc | deletion mutant FTdelta23 with 23 residues deleted from the C-terminus | Helicobacter pylori | |
2.4.1.214 | 0.78 | - |
LacNAc | deletion mutant FTdelta28 with 28 residues deleted from the C-terminus | Helicobacter pylori | |
2.4.1.214 | 0.89 | - |
LacNAc | deletion mutant FTdelta80 with 80 residues deleted from the C-terminus | Helicobacter pylori | |
2.4.1.214 | 8.92 | - |
LacNAc | deletion mutant FTdelta115 with 115 residues deleted from the C-terminus | Helicobacter pylori |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.214 | Helicobacter pylori | O30511 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.214 | 0.38 | - |
deletion mutant FTdelta115 with 115 residues deleted from the C-terminus, substrate LacNAc | Helicobacter pylori |
2.4.1.214 | 2.52 | - |
deletion mutant FTdelta23 with 23 residues deleted from the C-terminus, substrate LacNAc | Helicobacter pylori |
2.4.1.214 | 4.84 | - |
deletion mutant FTdelta28 with 28 residues deleted from the C-terminus, substrate LacNAc | Helicobacter pylori |
2.4.1.214 | 5.04 | - |
deletion mutant FTdelta38 with 38 residues deleted from the C-terminus, substrate LacNAc | Helicobacter pylori |
2.4.1.214 | 5.2 | - |
deletion mutant FTdelta80 with 80 residues deleted from the C-terminus, substrate LacNAc | Helicobacter pylori |
2.4.1.214 | 5.72 | - |
deletion mutant FTdelta45 with 45 residues deleted from the C-terminus, substrate LacNAc | Helicobacter pylori |
2.4.1.214 | 5.82 | - |
deletion mutant FTdelta66 with 66 residues deleted from the C-terminus, substrate LacNAc | Helicobacter pylori |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.214 | GDP-fucose + N-acetyllactosamine | - |
Helicobacter pylori | GDP + 3-fucosyl-N-acetyllactosamine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.214 | alpha1,3-fucosyltransferase | - |
Helicobacter pylori |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.214 | 25 | - |
glycosyltransferase assay | Helicobacter pylori |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.214 | 7.5 | - |
glycosyltransferase assay | Helicobacter pylori |