Any feedback?
Literature summary extracted from
- Flavin redox chemistry precedes substrate chlorination during the reaction of the flavin-dependent halogenase RebH (2006), Biochemistry, 45, 7904-7912.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.19.9 | - |
Lentzea aerocolonigenes |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.19.9 | additional information | - |
additional information | stopped-flow analysis of flavin intermediates | Lentzea aerocolonigenes |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.9 | additional information | Lentzea aerocolonigenes | first step in biosynthesis of rebeccamycin | ? | - |
? |  |
| 1.14.19.9 | tryptophan + FADH2 + O2 + Cl- + H+ | Lentzea aerocolonigenes | initial step of rebeccamycin biosynthesis | 7-chlorotryptophan + FAD + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.9 | Lentzea aerocolonigenes | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.19.9 | RebH is purified by size-exclusion chromatography | Lentzea aerocolonigenes |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.19.9 | tryptophan + FADH2 + chloride + O2 + H+ = 7-chloro-L-tryptophan + FAD + 2 H2O | FADH2 and O2 form an 4alpha-FAD-OOH intermediate that decays to 4alpha-FAD-OH. Tryptophan likely does not react directly with any flavin intermediate. Substrate chlorination occurs after completion of the flavin redox reactions | Lentzea aerocolonigenes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.9 | additional information | first step in biosynthesis of rebeccamycin | Lentzea aerocolonigenes | ? | - |
? | |
| 1.14.19.9 | tryptophan + FADH2 + O2 + Cl- + H+ | initial step of rebeccamycin biosynthesis | Lentzea aerocolonigenes | 7-chlorotryptophan + FAD + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.19.9 | halogenase | - |
Lentzea aerocolonigenes |
| 1.14.19.9 | RebH | - |
Lentzea aerocolonigenes |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.9 | 25 | - |
end-point assay for single-turnover reactions | Lentzea aerocolonigenes |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.19.9 | 0.012 | - |
FADH2 | 4°C | Lentzea aerocolonigenes | |
| 1.14.19.9 | 0.05 | - |
tryptophan | ranges from 0.02 s-1 to 0.05 s-1 for different preparations, 25°C | Lentzea aerocolonigenes | |
| 1.14.19.9 | 0.12 | - |
FADH2 | 25°C | Lentzea aerocolonigenes | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.9 | 7.5 | - |
end-point assay for single-turnover reactions | Lentzea aerocolonigenes |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.9 | FADH2 | - |
Lentzea aerocolonigenes | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
