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Literature summary extracted from

  • Heredia, V.V.; Thomson, J.; Nettleton, D.; Sun, S.
    Glucose-induced conformational changes in glucokinase mediate allosteric regulation: transient kinetic analysis (2006), Biochemistry, 45, 7553-7562.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.1 expression in Escherichia coli BL21 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.1 Y214C the glucose binding affinity is increased while its cooperativity is decreased Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.14
-
 ATP cosubstrate: glucose, wild-type enzyme Homo sapiens
2.7.1.1 0.23
-
 D-glucose mutant enzyme Y214C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Homo sapiens
-
 recombinantly expressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + 2-deoxy-D-glucose
-
 Homo sapiens ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-glucose
-
 Homo sapiens ADP + D-glucose 6-phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.1 29
-
 2-deoxy-D-glucose wild-type enzyme Homo sapiens
2.7.1.1 38
-
 D-glucose wild-type enzyme Homo sapiens
2.7.1.1 39
-
 D-glucose mutant enzyme Y214C Homo sapiens