Any feedback?
Literature summary extracted from
- Semisynthesis and characterization of mammalian thioredoxin reductase (2006), Biochemistry, 45, 5158-5170.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | expressed in Escherichia coli ER2566 cells | Mus musculus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | U489C | barely detectable activity towards thioredoxin and hydrogen peroxide | Mus musculus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | 6.6 | - |
thioredoxin | mutant enzyme U489C | Mus musculus |  |
| 1.8.1.9 | 6.6 | - |
hydrogen peroxide | semisynthetic enzyme with 91% content of selenium | Mus musculus | |
| 1.8.1.9 | 35 | - |
thioredoxin | semisynthetic enzyme with 63% content of selenium | Mus musculus | |
| 1.8.1.9 | 67.6 | - |
thioredoxin | semisynthetic enzyme with 91% content of selenium | Mus musculus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.9 | Mus musculus | Q9JLT4 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | chitin resin column chromatography and DEAE anion exchange column chromatography | Mus musculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.9 | thioredoxin + NADP+ | - |
Mus musculus | thioredoxin disulfide + NADPH | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | thioredoxin reductase | - |
Mus musculus |
| 1.8.1.9 | TR | - |
Mus musculus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | 0.02 | - |
thioredoxin | mutant enzyme U489C | Mus musculus | |
| 1.8.1.9 | 1.183 | - |
hydrogen peroxide | semisynthetic enzyme with 91% content of selenium | Mus musculus | |
| 1.8.1.9 | 6.08 | - |
hydrogen peroxide | semisynthetic enzyme with 91% content of selenium | Mus musculus | |
| 1.8.1.9 | 25 | - |
thioredoxin | semisynthetic enzyme with 63% content of selenium | Mus musculus | |
| 1.8.1.9 | 37 | - |
thioredoxin | semisynthetic enzyme with 91% content of selenium | Mus musculus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
