show Kinetic mechanism of deoxyadenosine kinase from Mycoplasma determined by surface plasmon resonance technology
Pol, E.; Wang, L.; Biochemistry 45, 513-522 (2006)
Data extracted from this reference:
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.76
0.01
-
deoxyadenosine
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.016
-
ATP
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.018
-
deoxyadenosine
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.019
-
deoxyguanosine
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.02
-
deoxyguanosine
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.021
-
CTP
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.077
-
TTP
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.121
-
deoxycytidine
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.135
-
UTP
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.146
-
deoxycytidine
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.76
Mycoplasma mycoides
-
subspecies Mycoplasma mycoides mycoides strain SC
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
ATP + deoxyadenosine
-
672159
Mycoplasma mycoides
ADP + dAMP
-
-
-
?
2.7.1.76
ATP + deoxycytidine
-
672159
Mycoplasma mycoides
ADP + dCMP
-
-
-
?
2.7.1.76
ATP + deoxyguanosine
-
672159
Mycoplasma mycoides
ADP + dGMP
-
-
-
?
2.7.1.76
CTP + deoxyadenosine
-
672159
Mycoplasma mycoides
CDP + dAMP
-
-
-
?
2.7.1.76
TTP + deoxyadenosine
-
672159
Mycoplasma mycoides
TDP + dAMP
-
-
-
?
2.7.1.76
UTP + deoxyadenosine
-
672159
Mycoplasma mycoides
UDP + dAMP
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.76
ATP
-
Mycoplasma mycoides
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.76
ATP
-
Mycoplasma mycoides
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.76
0.01
-
deoxyadenosine
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.016
-
ATP
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.018
-
deoxyadenosine
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.019
-
deoxyguanosine
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.02
-
deoxyguanosine
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.021
-
CTP
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.077
-
TTP
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.121
-
deoxycytidine
with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.135
-
UTP
with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
2.7.1.76
0.146
-
deoxycytidine
with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2
Mycoplasma mycoides
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.76
ATP + deoxyadenosine
-
672159
Mycoplasma mycoides
ADP + dAMP
-
-
-
?
2.7.1.76
ATP + deoxycytidine
-
672159
Mycoplasma mycoides
ADP + dCMP
-
-
-
?
2.7.1.76
ATP + deoxyguanosine
-
672159
Mycoplasma mycoides
ADP + dGMP
-
-
-
?
2.7.1.76
CTP + deoxyadenosine
-
672159
Mycoplasma mycoides
CDP + dAMP
-
-
-
?
2.7.1.76
TTP + deoxyadenosine
-
672159
Mycoplasma mycoides
TDP + dAMP
-
-
-
?
2.7.1.76
UTP + deoxyadenosine
-
672159
Mycoplasma mycoides
UDP + dAMP
-
-
-
?