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Literature summary extracted from

  • Li, M.; Binda, C.; Mattevi, A.; Edmondson, D.E.
    Functional role of the "aromatic cage" in human monoamine oxidase B: structures and catalytic properties of Tyr435 mutant proteins (2006), Biochemistry, 45, 4775-4784.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.4 expression of mutant enzymes in Pichia pastoris Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.3.4 sitting-drop vapor diffusion method, mutant enzymes Y435H, Y435F, Y435L, Y435W and wild-type enzyme in complex with p-nitrobenzylamine Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.4 Y435F kcat/KM for benzylamine is 21.3fold lower than wild-type value, kcat/KM for p-trifluoromethyl-benzylamine is 40fold lower than wild-type value, kcat/KM for p-nitrobenzylamine is 4.1fold lower than wild-type value, kcat/KM for phenylethylamine is 35.9fold lower than wild-type value, kcat/KM for p-nitrophenylethylamine is 8.3fold lower than wild-type value Homo sapiens
1.4.3.4 Y435H kcat/KM for benzylamine is 120fold lower than wild-type value, kcat/KM for p-trifluoromethyl-benzylamine is 13fold lower than wild-type value, kcat/KM for p-nitrobenzylamine is 4.4fold lower than wild-type value, kcat/KM for phenylethylamine is 1037fold lower than wild-type value, kcat/KM for p-nitrophenylethylamine is 45.2 fold lower than wild-type value Homo sapiens
1.4.3.4 Y435L kcat/KM for benzylamine is 128fold lower than wild-type value, kcat/KM for p-trifluoromethyl-benzylamine is 58fold lower than wild-type value, kcat/KM for phenylethylamine is 15.2fold lower than wild-type value, kcat/KM for p-nitrophenylethylamine is 2.7fold lower than wild-type value Homo sapiens
1.4.3.4 Y435W kcat/KM for benzylamine is 9524fold lower than wild-type value, kcat/KM for phenylethylamine is 13889fold lower than wild-type value, kcat/KM for p-nitrophenylethylamine is 23fold lower than wild-type value Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.4 p-nitrobenzylamine substrate for wild-type enzyme, competitive inhibitor for mutant enzyme Y435L Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.4 0.005
-
 p-nitrophenylethylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
1.4.3.4 0.013
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
1.4.3.4 0.016
-
 2-Phenylethylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
1.4.3.4 0.05
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
1.4.3.4 0.05
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
1.4.3.4 0.05
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
1.4.3.4 0.09
-
 p-nitrobenzylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
1.4.3.4 0.13
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
1.4.3.4 0.14
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435W Homo sapiens
1.4.3.4 0.15
-
 benzylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
1.4.3.4 0.22
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
1.4.3.4 0.29
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
1.4.3.4 0.49
-
 p-nitrobenzylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
1.4.3.4 0.68
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435W Homo sapiens
1.4.3.4 0.76
-
 p-nitrobenzylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
1.4.3.4 0.99
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
1.4.3.4 1.15
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
1.4.3.4 1.4
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
1.4.3.4 2
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
1.4.3.4 2.49
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
1.4.3.4 7.2
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
1.4.3.4 8.8
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435W Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.4 Homo sapiens P27338
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.4
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.4 2-phenylethylamine + H2O + O2
-
 Homo sapiens 2-phenylethanal + NH3 + H2O2
-
 ?
1.4.3.4 benzylamine + H2O + O2
-
 Homo sapiens benzaldehyde + NH3 + H2O2
-
 ?
1.4.3.4 p-nitrobenzylamine + H2O + O2 poor substrate for wild-type enzyme and mutant enzymes Y435H and Y435F Homo sapiens 4-nitrobenzaldehyde + NH3 + H2O2
-
 ?
1.4.3.4 p-nitrophenylethylamine + H2O + O2 good substrate for wild-type and mutant enzymes Y435H, Y435F, Y435L and Y435W Homo sapiens 4-nitrophenylacetaldehyde + NH3 + H2O2
-
 ?
1.4.3.4 p-trifluoromethyl-benzylamine + H2O + O2
-
 Homo sapiens 4-trifluoromethylbenzaldehyde + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.4 monoamine oxidase B
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.4 0.012
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435W Homo sapiens
1.4.3.4 0.028
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435W Homo sapiens
1.4.3.4 0.066
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
1.4.3.4 0.185
-
 p-nitrobenzylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
1.4.3.4 0.23
-
 p-nitrobenzylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
1.4.3.4 0.38
-
 p-nitrobenzylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
1.4.3.4 0.455
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
1.4.3.4 0.647
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
1.4.3.4 0.79
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
1.4.3.4 0.87
-
 2-Phenylethylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
1.4.3.4 1.33
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
1.4.3.4 1.33
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
1.4.3.4 1.8
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
1.4.3.4 1.8
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
1.4.3.4 1.8
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens
1.4.3.4 1.85
-
 p-nitrophenylethylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
1.4.3.4 1.99
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
1.4.3.4 2.23
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435F Homo sapiens
1.4.3.4 2.24
-
 p-nitrophenylethylamine pH 7.5, 25°C, mutant enzyme Y435W Homo sapiens
1.4.3.4 2.85
-
 p-trifluoromethyl-benzylamine pH 7.5, 25°C, mutant enzyme Y435H Homo sapiens
1.4.3.4 3.82
-
 2-Phenylethylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
1.4.3.4 5
-
 benzylamine pH 7.5, 25°C, wild-type enzyme Homo sapiens
1.4.3.4 6.08
-
 benzylamine pH 7.5, 25°C, mutant enzyme Y435L Homo sapiens