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Literature summary extracted from

  • Sakai, A.; Xiang, D.F.; Xu, C.; Song, L.; Yew, W.S.; Raushel, F.M.; Gerlt, J.A.
    Evolution of enzymatic activities in the enolase superfamily: N-succinylamino acid racemase and a new pathway for the irreversible conversion of D- to L-amino acids (2006), Biochemistry, 45, 4455-4462.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.10 protein is expressed in Escherichia coli strain BL21 Geobacillus kaustophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.10 additional information
-
 N-Succinyl-L-Glu low specific rotation prevented kinetic analysis Geobacillus kaustophilus
5.1.1.10 additional information
-
 N-Succinyl-L-Asn low specific rotation prevented kinetic analysis Geobacillus kaustophilus
5.1.1.10 additional information
-
 N-Succinyl-L-Gln low specific rotation prevented kinetic analysis Geobacillus kaustophilus
5.1.1.10 additional information
-
 N-Succinyl-L-Asp low specific rotation prevented kinetic analysis Geobacillus kaustophilus
5.1.1.10 additional information
-
 N-Succinyl-L-Lys low specific rotation prevented kinetic analysis Geobacillus kaustophilus
5.1.1.10 additional information
-
 N-Succinyl-L-Arg low specific rotation prevented kinetic analysis Geobacillus kaustophilus
5.1.1.10 additional information
-
 N-Succinyl-L-Thr low specific rotation prevented kinetic analysis Geobacillus kaustophilus
5.1.1.10 additional information
-
 N-Succinyl-L-Cys low specific rotation prevented kinetic analysis Geobacillus kaustophilus
5.1.1.10 additional information
-
 additional information no activity with N-Succinyl-L-Pro Geobacillus kaustophilus
5.1.1.10 0.8
-
 N-succinyl-L-Phe
-
 Geobacillus kaustophilus
5.1.1.10 1.3
-
 N-Succinyl-L-Leu
-
 Geobacillus kaustophilus
5.1.1.10 2.5
-
 N-succinyl-L-Val
-
 Geobacillus kaustophilus
5.1.1.10 2.6
-
 N-succinyl-L-Ala
-
 Geobacillus kaustophilus
5.1.1.10 2.6
-
 N-succinyl-L-Tyr
-
 Geobacillus kaustophilus
5.1.1.10 3
-
 N-succinyl-L-Ser
-
 Geobacillus kaustophilus
5.1.1.10 3.6
-
 N-Succinyl-L-Met
-
 Geobacillus kaustophilus
5.1.1.10 3.7
-
 N-succinyl-L-Ile
-
 Geobacillus kaustophilus
5.1.1.10 3.8
-
 N-succinyl-L-His
-
 Geobacillus kaustophilus
5.1.1.10 7.1
-
 N-succinyl-L-Trp
-
 Geobacillus kaustophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.1.10 Mn2+ assay with 0.1 mM MnCl2 Geobacillus kaustophilus

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.10 Geobacillus kaustophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.10 protein is purified by applying to a diethylaminoethyl sepharose fast flow column and a phenyl sepharose 6 fast flow column Geobacillus kaustophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.10 N-succinyl-L-Arg
-
 Geobacillus kaustophilus N-succinyl-D-Arg
-
 r
5.1.1.10 N-succinyl-L-Asn
-
 Geobacillus kaustophilus N-succinyl-D-Asn
-
 r
5.1.1.10 N-succinyl-L-Asp
-
 Geobacillus kaustophilus N-succinyl-D-Asp
-
 r
5.1.1.10 N-succinyl-L-Gln
-
 Geobacillus kaustophilus N-succinyl-D-Gln
-
 r
5.1.1.10 N-succinyl-L-Glu
-
 Geobacillus kaustophilus N-succinyl-D-Glu
-
 r
5.1.1.10 N-succinyl-L-His
-
 Geobacillus kaustophilus N-succinyl-D-His
-
 r
5.1.1.10 N-succinyl-L-Ile
-
 Geobacillus kaustophilus N-succinyl-D-Ile
-
 r
5.1.1.10 N-succinyl-L-Leu
-
 Geobacillus kaustophilus N-succinyl-D-Leu
-
 r
5.1.1.10 N-succinyl-L-Lys
-
 Geobacillus kaustophilus N-succinyl-D-Lys
-
 r
5.1.1.10 N-succinyl-L-Met
-
 Geobacillus kaustophilus N-succinyl-D-Met
-
 r
5.1.1.10 N-succinyl-L-phenylalanine
-
 Geobacillus kaustophilus N-succinyl-D-phenylalanine
-
 ?
5.1.1.10 N-succinyl-L-Ser
-
 Geobacillus kaustophilus N-succinyl-D-Ser
-
 r
5.1.1.10 N-succinyl-L-Trp
-
 Geobacillus kaustophilus N-succinyl-D-Trp
-
 r
5.1.1.10 N-succinyl-L-Tyr
-
 Geobacillus kaustophilus N-succinyl-D-Tyr
-
 r
5.1.1.10 N-succinyl-L-Val
-
 Geobacillus kaustophilus N-succinyl-D-Val
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.1.10 N-succinylamino acid racemase
-
 Geobacillus kaustophilus
5.1.1.10 NSAR
-
 Geobacillus kaustophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.10 25
-
 assay at Geobacillus kaustophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.1.10 additional information
-
 additional information N-Succinyl-L-Cys, low specific rotation prevented kinetic analysis Geobacillus kaustophilus
5.1.1.10 additional information
-
 additional information N-Succinyl-L-Thr, low specific rotation prevented kinetic analysis Geobacillus kaustophilus
5.1.1.10 additional information
-
 additional information no activity with N-Succinyl-L-Pro Geobacillus kaustophilus
5.1.1.10 0.014
-
 N-Succinyl-L-Arg
-
 Geobacillus kaustophilus
5.1.1.10 0.071
-
 N-Succinyl-L-Asp
-
 Geobacillus kaustophilus
5.1.1.10 0.073
-
 N-Succinyl-L-Glu
-
 Geobacillus kaustophilus
5.1.1.10 2
-
 N-succinyl-L-His
-
 Geobacillus kaustophilus
5.1.1.10 2.1
-
 N-Succinyl-L-Lys
-
 Geobacillus kaustophilus
5.1.1.10 2.5
-
 N-Succinyl-L-Asn
-
 Geobacillus kaustophilus
5.1.1.10 7.9
-
 N-succinyl-L-Trp
-
 Geobacillus kaustophilus
5.1.1.10 9
-
 N-Succinyl-L-Gln
-
 Geobacillus kaustophilus
5.1.1.10 17
-
 N-Succinyl-L-Leu
-
 Geobacillus kaustophilus
5.1.1.10 19
-
 N-succinyl-L-Phe
-
 Geobacillus kaustophilus
5.1.1.10 35
-
 N-succinyl-L-Ile
-
 Geobacillus kaustophilus
5.1.1.10 45
-
 N-succinyl-L-Ser
-
 Geobacillus kaustophilus
5.1.1.10 52
-
 N-succinyl-L-Tyr
-
 Geobacillus kaustophilus
5.1.1.10 53
-
 N-Succinyl-L-Met
-
 Geobacillus kaustophilus
5.1.1.10 89
-
 N-succinyl-L-Ala
-
 Geobacillus kaustophilus
5.1.1.10 92
-
 N-succinyl-L-Val
-
 Geobacillus kaustophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.10 7.9
-
 assay at Geobacillus kaustophilus