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Literature summary extracted from

  • Mani, R.S.; Usova, E.V.; Cass, C.E.; Eriksson, S.
    Fluorescence energy transfer studies of human deoxycytidine kinase: role of cysteine 185 in the conformational changes that occur upon substrate binding (2006), Biochemistry, 45, 3534-3541.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.74 native and mutant proteins expressed as His-tag fusion proteins in Escherichia coli BL21(DE3)pLysS Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.74 C146A very low activity Homo sapiens
2.7.1.74 C185A lower efficiency with dCyd as the substrate with ATP und UTP, nearly 2-fold higher efficiency than wild-type dCK with dAdo as substrate and UTP as the phosphate donor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 0.011
-
 UTP wild type enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6 Homo sapiens
2.7.1.74 0.017
-
 UTP C185A mutant enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6 Homo sapiens
2.7.1.74 0.019
-
 ATP wild type enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6 Homo sapiens
2.7.1.74 0.02
-
 UTP C185A mutant enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6 Homo sapiens
2.7.1.74 0.025
-
 ATP C185A mutant enzyme with 2'-deoxycytidine as phosphate acceptor, pH 7.6 Homo sapiens
2.7.1.74 0.04
-
 UTP wild type enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6 Homo sapiens
2.7.1.74 0.087
-
 ATP C185A mutant enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6 Homo sapiens
2.7.1.74 0.097
-
 ATP wild type enzyme with 2'-deoxyadenosine as phosphate acceptor, pH 7.6 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.74 additional information Homo sapiens phosphorylates both pyrimidine and purine deoxynucleosides, including numerous nucleoside analogue anticancer and antiviral prodrugs ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens P27707
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74 recombinant enzymes using His-tag Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxyadenosine
-
 Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.74 ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.74 additional information phosphorylates both pyrimidine and purine deoxynucleosides, including numerous nucleoside analogue anticancer and antiviral prodrugs Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.74 dCK
-
 Homo sapiens