EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.60 | expressed in Sf9 insect cells | Homo sapiens |
5.1.3.14 | expression in Insect Cells and supercompetent InvalphaF' Escherichia coli cells (Invitrogen) | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.60 | A524V | 30% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | A630T | 40% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | A631T | 75% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | A631V | 15% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | A631V | 65% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | C13S | 105% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | C303V | 60% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | C303X | no ManNAc kinase activity | Homo sapiens |
2.7.1.60 | D176V | 85% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | D378Y | 45% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | F528C | 35% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | F537I | 60% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | G576E | 15% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | G708S | 5% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | H132Q | 50% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | I200F | 75% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | I472T | 5% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | I587T | 35% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | M712T | 70% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | N519S | 20% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | R11W | 40% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | R177C | 80% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | V331A | 115% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
2.7.1.60 | V572L | 10% ManNAc kinase activity of the wild type enzyme | Homo sapiens |
5.1.3.14 | C303V | exhibited almost no reduction in epimerase activity | Escherichia coli |
5.1.3.14 | C303X | the C303X protein does not display any enzymatic activity | Escherichia coli |
5.1.3.14 | D378Y | 60% reduction of epimerase activity | Escherichia coli |
5.1.3.14 | I200F | exhibited almost no reduction in epimerase activity | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.3.14 | UDP-GlcNAc | Escherichia coli | biosynthetic pathway of sialic acid | ManNAc + UDP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.60 | Homo sapiens | - |
- |
- |
5.1.3.14 | Escherichia coli | P27828 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.60 | Ni-NTA column chromatography and PD-10 gel column filtration | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.60 | ATP + N-acetyl-D-mannosamine | - |
Homo sapiens | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? | |
5.1.3.14 | UDP-GlcNAc | biosynthetic pathway of sialic acid | Escherichia coli | ManNAc + UDP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.1.3.14 | dimer | - |
Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.60 | UDP-GlcNAc 2-epimerase/ManNAc kinase | - |
Homo sapiens |
5.1.3.14 | UDP-GlcNAc 2-epimerase | - |
Escherichia coli |
5.1.3.14 | UDP-N-acetylglucosamine 2-epimerase | - |
Escherichia coli |