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Literature summary extracted from

  • Penner, J.; Mantey, L.R.; Elgavish, S.; Ghaderi, D.; Cirak, S.; Berger, M.; Krause, S.; Lucka, L.; Voit, T.; Mitrani-Rosenbaum, S.; Hinderlich, S.
    Influence of UDP-GlcNAc 2-epimerase/ManNAc kinase mutant proteins on hereditary inclusion body myopathy (2006), Biochemistry, 45, 2968-2977.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.60 expressed in Sf9 insect cells Homo sapiens
5.1.3.14 expression in Insect Cells and supercompetent InvalphaF' Escherichia coli cells (Invitrogen) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.60 A524V 30% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 A630T 40% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 A631T 75% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 A631V 15% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 A631V 65% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 C13S 105% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 C303V 60% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 C303X no ManNAc kinase activity Homo sapiens
2.7.1.60 D176V 85% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 D378Y 45% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 F528C 35% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 F537I 60% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 G576E 15% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 G708S 5% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 H132Q 50% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 I200F 75% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 I472T 5% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 I587T 35% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 M712T 70% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 N519S 20% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 R11W 40% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 R177C 80% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 V331A 115% ManNAc kinase activity of the wild type enzyme Homo sapiens
2.7.1.60 V572L 10% ManNAc kinase activity of the wild type enzyme Homo sapiens
5.1.3.14 C303V exhibited almost no reduction in epimerase activity Escherichia coli
5.1.3.14 C303X the C303X protein does not display any enzymatic activity Escherichia coli
5.1.3.14 D378Y 60% reduction of epimerase activity Escherichia coli
5.1.3.14 I200F exhibited almost no reduction in epimerase activity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.14 UDP-GlcNAc Escherichia coli biosynthetic pathway of sialic acid ManNAc + UDP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.60 Homo sapiens
-
-
-
5.1.3.14 Escherichia coli P27828
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.60 Ni-NTA column chromatography and PD-10 gel column filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.60 ATP + N-acetyl-D-mannosamine
-
Homo sapiens ADP + N-acetyl-D-mannosamine 6-phosphate
-
?
5.1.3.14 UDP-GlcNAc biosynthetic pathway of sialic acid Escherichia coli ManNAc + UDP
-
?

Subunits

EC Number Subunits Comment Organism
5.1.3.14 dimer
-
Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.1.60 UDP-GlcNAc 2-epimerase/ManNAc kinase
-
Homo sapiens
5.1.3.14 UDP-GlcNAc 2-epimerase
-
Escherichia coli
5.1.3.14 UDP-N-acetylglucosamine 2-epimerase
-
Escherichia coli