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Literature summary extracted from

  • Purdy, M.M.; Koo, L.S.; Montellano, P.R.; Klinman, J.P.
    Mechanism of O2 activation by cytochrome P450cam studied by isotope effects and transient state kinetics (2006), Biochemistry, 45, 15793-15806.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.1 additional information increasing viscosity of the assay mixture, e.g. by addition of glucose or sucrose, reduces kcat Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.1 additional information
-
 additional information equilibrium isotope effect on O2 binding, isotope effects and transient-state kinetics, electron transfer kinetics, steady-state kinetics, recombinant His6-tagged enzyme, overview Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.1 Fe2+ a cytochrome P450 monooxygenase Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin Pseudomonas putida
-
 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.1 (+)-camphor + reduced putidaredoxin + O2 = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O mechanism of O2 activation: binding of O2 to ferrous P450cam to yield the ferric-superoxo form, oxyP450cam, followed by an irreversible, long-range electron transfer from putidaredoxin to reduce the oxyP450cam, camphor is bound to all enzyme forms, overview Pseudomonas putida
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?
1.14.15.1 (+)-camphor + reduced putidaredoxin + O2
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.14.15.1 cytochrome p450cam
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.1 25
-
 assay at Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.15.1 39
-
 (+)-Camphor pH 7.9, 25°C, recombinant His6-tagged enzyme Pseudomonas putida
1.14.15.1 47
-
 (+)-Camphor pH 6.9, 25°C, recombinant His6-tagged enzyme Pseudomonas putida
1.14.15.1 51
-
 (+)-Camphor pH 7.4, 25°C, recombinant His6-tagged enzyme Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.1 7.4
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.15.1 additional information
-
 pH profile Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 putidaredoxin electron transfer from putidaredoxin to the ferric-superoxo enzyme form, overview Pseudomonas putida