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Literature summary extracted from

  • Ma, J.J.; Zhao, M.W.; Wang, E.D.
    Split leucine-specific domain of leucyl-tRNA synthetase from the hyperthermophilic bacterium Aquifex aeolicus (2006), Biochemistry, 45, 14809-14816.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.4 individual expression of His-tagged alpha- and beta-subunits in Escherichia coli strain BL21(DE3) Aquifex aeolicus

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.4 K599A site-directed mutagenesis, the mutation of the residue in the alpha-subunit involved in catalysis only slightly affects the enzyme activity Aquifex aeolicus
6.1.1.4 K599A/K600A site-directed mutagenesis, the mutation of the residue in the alpha-subunit involved in catalysis only slightly affects the enzyme activity Aquifex aeolicus
6.1.1.4 K599A/K600A/K605A/K606A site-directed mutagenesis, the mutation of the residue in the alpha-subunit involved in catalysis only slightly affects the enzyme activity Aquifex aeolicus
6.1.1.4 K600A site-directed mutagenesis, the mutation of the residue in the alpha-subunit involved in catalysis only slightly affects the enzyme activity Aquifex aeolicus
6.1.1.4 K605A site-directed mutagenesis, the mutation of the residue in the alpha-subunit involved in catalysis only slightly affects the enzyme activity Aquifex aeolicus
6.1.1.4 K605A/K606A site-directed mutagenesis, the mutation of the residue in the alpha-subunit involved in catalysis only slightly affects the enzyme activity Aquifex aeolicus
6.1.1.4 K606A site-directed mutagenesis, the mutation of the residue in the alpha-subunit involved in catalysis only slightly affects the enzyme activity Aquifex aeolicus
6.1.1.4 additional information construction of diverse truncation mutants, ATP-diphosphate-exchange and aminoacylation activities, and ability to charge minihelixLeu of alpha and beta subunit mutants, overview Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 additional information
-
additional information kinetics of recombinant trunacted mutants, overview Aquifex aeolicus
6.1.1.4 0.0003
-
tRNALeuGAG pH 6.8, 65°C, recombinant wild-type enzyme Aquifex aeolicus
6.1.1.4 0.006
-
L-leucine pH 6.8, 65°C, recombinant wild-type enzyme Aquifex aeolicus
6.1.1.4 0.1123
-
ATP pH 6.8, 65°C, recombinant wild-type enzyme Aquifex aeolicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.4 Mg2+
-
Aquifex aeolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu Aquifex aeolicus
-
AMP + diphosphate + L-leucyl-tRNALeu
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Aquifex aeolicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4 recombinant His-tagged alpha- and beta-subunits from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
?
6.1.1.4 ATP + L-leucine + tRNALeu recombinant tRNALeu substrate, two peptides of eight and nine amino acid residues in the domain located in the alpha subunit are essential for the enzyme’s activity Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
?
6.1.1.4 ATP + L-leucine + tRNALeuGAG
-
Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeuGAG
-
?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 dimer (alphabeta) heterodimer with a large extra domain, the leucine-specific domain, inserted into the catalytic domain, the subunit split site is exactly in the middle of the leucine-specific domain and may have a unique function, two peptides of eight and nine amino acid residues in the domain located in the alpha subunit are essential for the enzyme’s activity Aquifex aeolicus

Synonyms

EC Number Synonyms Comment Organism
6.1.1.4 Leucyl-tRNA synthetase
-
Aquifex aeolicus
6.1.1.4 LeuRS
-
Aquifex aeolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.4 37 65 broad temperature optimum Aquifex aeolicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 1.4
-
L-leucine pH 6.8, 65°C, recombinant wild-type enzyme Aquifex aeolicus
6.1.1.4 1.5
-
tRNALeuGAG pH 6.8, 65°C, recombinant wild-type enzyme Aquifex aeolicus
6.1.1.4 1.81
-
ATP pH 6.8, 65°C, recombinant wild-type enzyme Aquifex aeolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.4 6.8
-
assay at Aquifex aeolicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.4 ATP
-
Aquifex aeolicus