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Literature summary extracted from

  • Catalano-Dupuy, D.L.; Orecchia, M.; Rial, D.V.; Ceccarelli, E.A.
    Reduction of the pea ferredoxin-NADP(H) reductase catalytic efficiency by the structuring of a carboxyl-terminal artificial metal binding site (2006), Biochemistry, 45, 13899-13909.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.18.1.2 additional information addition of an artificial metal binding site of nine amino acids, including four His residues, to the C-terminal Tyr308 residue. The additional structure binds Zn2+ or Co2+ and significantly reduces the catalytic efficiency of the enzyme by decreasing the kcat value. In absence of Zn2+, Km value of NADPH and Kd value for NADP+ are increased 2 to 3 times Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.0012
-
 NADPH wild-type, 30°C, pH 8.0 Pisum sativum
1.18.1.2 0.0024
-
 NADPH mutant bearing an artificial metal binding site of nine amino acids at the C-terminus, absence of Zn2+, 30°C, pH 8.0 Pisum sativum
1.18.1.2 0.0026
-
 NADPH mutant bearing an artificial metal binding site of nine amino acids at the C-terminus, presence of Zn2+, 30°C, pH 8.0 Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Pisum sativum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Pisum sativum NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 2.2
-
 NADPH wild-type, 30°C, pH 8.0 Pisum sativum
1.18.1.2 10.1
-
 NADPH mutant bearing an artificial metal binding site of nine amino acids at the C-terminus, presence of Zn2+, 30°C, pH 8.0 Pisum sativum
1.18.1.2 73.5
-
 NADPH mutant bearing an artificial metal binding site of nine amino acids at the C-terminus, absence of Zn2+, 30°C, pH 8.0 Pisum sativum
1.18.1.2 74.1
-
 NADPH wild-type, 30°C, pH 8.0 Pisum sativum