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Literature summary extracted from

  • Francis, K.; Gadda, G.
    Probing the chemical steps of nitroalkane oxidation catalyzed by 2-nitropropane dioxygenase with solvent viscosity, pH, and substrate kinetic isotope effects (2006), Biochemistry, 45, 13889-13898.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.16 m-Nitrobenzoate
-
 Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.16 0.005
-
 O2 with ethyl nitronate at pH 8.0 and 30°C Neurospora crassa
1.13.12.16 0.005
-
 O2 with nitroethane at pH 9.0 and 30°C Neurospora crassa
1.13.12.16 0.005
-
 O2 with ethyl nitronate at pH 8.0 and 30°C; with nitroethane at pH 9.0 and 30°C Neurospora crassa
1.13.12.16 0.01
-
 O2 with nitroethane at pH 6.0 and 30°C Neurospora crassa
1.13.12.16 0.02
-
 O2 with either ethyl nitronate or nitroethane at pH 6.0 and 30°C Neurospora crassa
1.13.12.16 0.045
-
 O2 with ethyl nitronate at pH 9.0 and 30°C Neurospora crassa
1.13.12.16 3.4
-
 ethyl nitronate at pH 8.0 and 30°C Neurospora crassa
1.13.12.16 5.1
-
 ethyl nitronate at pH 6.0 and 30°C Neurospora crassa
1.13.12.16 9.5
-
 ethyl nitronate at pH 9.0 and 30°C Neurospora crassa
1.13.12.16 13
-
 nitroethane at pH 9.0 and 30°C Neurospora crassa
1.13.12.16 19
-
 nitroethane at pH 8.0 and 30°C Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.16 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.16
-
 Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.16 ethyl nitronate + O2
-
 Neurospora crassa ? + nitrite
-
 ?
1.13.12.16 ethyl nitronate + O2 anionic form of the substrate Neurospora crassa ? + nitrite
-
 ?
1.13.12.16 nitroethane + O2 neutral form of the substrate Neurospora crassa acetaldehyde + HNO2
-
 ?
1.13.12.16 nitroethane + O2 + H2O
-
 Neurospora crassa ethanal + nitrite + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.16 2-nitropropane dioxygenase
-
 Neurospora crassa
1.13.12.16 NAO
-
 Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.16 30
-
-
 Neurospora crassa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.12.16 4.9
-
 nitroethane at pH 9.0 and 30°C Neurospora crassa
1.13.12.16 11
-
 nitroethane at pH 8.0 and 30°C Neurospora crassa
1.13.12.16 25
-
 ethyl nitronate at pH 9.0 and 30°C Neurospora crassa
1.13.12.16 57
-
 ethyl nitronate at pH 8.0 and 30°C Neurospora crassa
1.13.12.16 130
-
 ethyl nitronate at pH 6.0 and 30°C Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.16 8.5
-
-
 Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.12.16 6 9 investigation of the pH effects on the kcat/Km-values Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.16 FMN dependent Neurospora crassa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.12.16 additional information
-
 additional information investigation of the pH effects on the kcat/Km-values Neurospora crassa
1.13.12.16 0.04
-
 nitroethane at pH 6.0 and 30°C Neurospora crassa
1.13.12.16 0.375
-
 nitroethane at pH 9.0 and 30°C Neurospora crassa
1.13.12.16 0.56
-
 nitroethane at pH 8.0 and 30°C Neurospora crassa
1.13.12.16 2.5
-
 ethyl nitronate at pH 9.0 and 30°C Neurospora crassa
1.13.12.16 16.9
-
 ethyl nitronate at pH 8.0 and 30°C Neurospora crassa
1.13.12.16 25.4
-
 ethyl nitronate at pH 6.0 and 30°C Neurospora crassa