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Literature summary extracted from

  • Xu, H.; West, A.H.; Cook, P.F.
    Overall kinetic mechanism of saccharopine dehydrogenase from Saccharomyces cerevisiae (2006), Biochemistry, 45, 12156-12166.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.7 alpha-ketoglutarate substrate inhibition, uncompetitive versus NADH Saccharomyces cerevisiae
1.5.1.7 L-lysine substrate inhibition, competitive versus NADH Saccharomyces cerevisiae
1.5.1.7 additional information detailed analysis of inhibition patterns for product and dead end inhibitors Saccharomyces cerevisiae
1.5.1.7 saccharopine product inhibition, uncompetitive versus NADH Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.7 0.019
-
 NADH 25°C, pH 7.0 Saccharomyces cerevisiae
1.5.1.7 0.9
-
 NAD+ 25°C, pH 7.0 Saccharomyces cerevisiae
1.5.1.7 1.1
-
 L-lysine 25°C, pH 7.0 Saccharomyces cerevisiae
1.5.1.7 6.7
-
 N6-(L-1,3-dicarboxypropyl)-L-lysine 25°C, pH 7.0 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.7 Saccharomyces cerevisiae
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O = L-lysine + 2-oxoglutarate + NADH + H+ ordered addition of NAD followed by saccharopine in the physiologic reaction direction. In the opposite direction, NADH adds to the enzyme first, while there is no preference for the order of binding of alpha-ketoglutarate and lysine Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.7 L-lysine + 2-oxoglutarate + NADH
-
 Saccharomyces cerevisiae N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
 ?
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
 Saccharomyces cerevisiae L-lysine + 2-oxoglutarate + NADH
-
 r

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.7 27.8
-
 L-lysine 25°C, pH 7.0 Saccharomyces cerevisiae
1.5.1.7 28
-
 alpha-ketoglutarate 25°C, pH 7.0 Saccharomyces cerevisiae