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Literature summary extracted from

  • Ingram-Smith, C.; Woods, B.I.; Smith, K.S.
    Characterization of the acyl substrate binding pocket of acetyl-CoA synthetase (2006), Biochemistry, 45, 11482-11490.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.1 expression in Escherichia coli Methanothermobacter thermautotrophicus

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.1 I312A kcat/Km for acetate is 15fold lower than wild-type value, kcat/Km for propionate is 5.2fold lower than wild-type value. Mutant enzyme shows activity with butyrate Methanothermobacter thermautotrophicus
6.2.1.1 T313K Km for acetate is 170fold higher than wild-type value, kcat for acetate is 34fold lower than wild-type value Methanothermobacter thermautotrophicus
6.2.1.1 T313V kcat/Km for acetate is 2.5fold lower than wild-type value, kcat/Km for propionate is identical to wild-type value Methanothermobacter thermautotrophicus
6.2.1.1 V388A kcat/Km for acetate is 6.9fold lower than wild-type value, kcat/Km for propionate is 2.5fold higher than wild-type value, mutant enzyme shows activity with wild-type enzyme Methanothermobacter thermautotrophicus
6.2.1.1 V388G kcat/Km for acetate is 93fold lower than wild-type value, kcat/Km for propionate is 26fold lower than wild-type value Methanothermobacter thermautotrophicus
6.2.1.1 W416G kcat/Km for acetate is 71.5fold lower than wild-type value, kcat/Km for propionate is 13fold lower than wild-type value, mutant enzyme shows activity with: butyrate, valerate, hexanoate, heptanoate, octanoate, 4-methylvalerate and 3-methylvalerate Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.1 0.18
-
 CoA 65°C, mutant enzyme I312A Methanothermobacter thermautotrophicus
6.2.1.1 0.18
-
 CoA 65°C, mutant enzyme V388G Methanothermobacter thermautotrophicus
6.2.1.1 0.19
-
 CoA 65°C, wild-type enzyme Methanothermobacter thermautotrophicus
6.2.1.1 0.35
-
 CoA 65°C, mutant enzyme V388A Methanothermobacter thermautotrophicus
6.2.1.1 0.43
-
 CoA 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 0.46
-
 Butyrate 65°C, mutant enzyme T313V Methanothermobacter thermautotrophicus
6.2.1.1 0.65
-
 ATP 65°C, mutant enzyme T313V Methanothermobacter thermautotrophicus
6.2.1.1 0.7
-
 CoA 65°C, mutant enzyme T313V Methanothermobacter thermautotrophicus
6.2.1.1 2.3
-
 ATP 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 2.6
-
 ATP 65°C, mutant enzyme V388A Methanothermobacter thermautotrophicus
6.2.1.1 3.3
-
 ATP 65°C, wild-type enzyme Methanothermobacter thermautotrophicus
6.2.1.1 3.4
-
 ATP 65°C, mutant enzyme T313K Methanothermobacter thermautotrophicus
6.2.1.1 3.5
-
 acetate 65°C, wild-type enzyme Methanothermobacter thermautotrophicus
6.2.1.1 4
-
 ATP 65°C, mutant enzyme V388G Methanothermobacter thermautotrophicus
6.2.1.1 4.1
-
 propionate 65°C, mutant enzyme V388A Methanothermobacter thermautotrophicus
6.2.1.1 4.5
-
 propionate 65°C, mutant enzyme T313V Methanothermobacter thermautotrophicus
6.2.1.1 6.1
-
 hexanoate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 6.6
-
 ATP 65°C, mutant enzyme I312A Methanothermobacter thermautotrophicus
6.2.1.1 7.6
-
 4-methylvalerate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 10.2
-
 Heptanoate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 11.1
-
 valerate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 13.2
-
 acetate 65°C, mutant enzyme V388A Methanothermobacter thermautotrophicus
6.2.1.1 18.1
-
 Octanoate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 24.6
-
 acetate 65°C, mutant enzyme I312A Methanothermobacter thermautotrophicus
6.2.1.1 29.2
-
 2-methylvalerate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 36.5
-
 propionate 65°C, wild-type enzyme Methanothermobacter thermautotrophicus
6.2.1.1 39.2
-
 Butyrate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 128.6
-
 propionate 65°C, mutant enzyme V388G Methanothermobacter thermautotrophicus
6.2.1.1 132.1
-
 acetate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 151.9
-
 Butyrate 65°C, mutant enzyme V388A Methanothermobacter thermautotrophicus
6.2.1.1 164.4
-
 acetate 65°C, mutant enzyme V388G Methanothermobacter thermautotrophicus
6.2.1.1 188.8
-
 propionate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 596
-
 acetate 65°C, mutant enzyme T313K Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.1 Methanothermobacter thermautotrophicus
-
-
-
6.2.1.1 Methanothermobacter thermautotrophicus Z245
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.1
-
 Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.1 ATP + 2-methylvalerate + CoA
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + 2-methylvaleryl-CoA
-
 ?
6.2.1.1 ATP + 3-methylvalerate + CoA mutant enzyme W416G catalyzes the reaction, no activity with wild-type enzyme Methanothermobacter thermautotrophicus AMP + diphosphate + 3-methylvaleryl-CoA
-
 ?
6.2.1.1 ATP + 4-methylvalerate + CoA mutant enzyme W416G catalyzes the reaction, no activity with wild-type enzyme Methanothermobacter thermautotrophicus AMP + diphosphate + 4-methylvaleryl-CoA
-
 ?
6.2.1.1 ATP + acetate + CoA
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.1 ATP + acetate + CoA ATP in form of MgATP2- Methanothermobacter thermautotrophicus AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.1 ATP + acetate + CoA
-
 Methanothermobacter thermautotrophicus Z245 AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.1 ATP + acetate + CoA ATP in form of MgATP2- Methanothermobacter thermautotrophicus Z245 AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.1 ATP + butyrate + CoA mutant enzymes I312A and W416G catalyzes the reaction, no activity with wild-type enzyme Methanothermobacter thermautotrophicus AMP + diphosphate + butyryl-CoA
-
 ?
6.2.1.1 ATP + butyrate + CoA mutant enzymes I312A and W416G catalyzes the reaction, no activity with wild-type enzyme Methanothermobacter thermautotrophicus Z245 AMP + diphosphate + butyryl-CoA
-
 ?
6.2.1.1 ATP + heptanoate + CoA mutant enzyme W416G catalyzes the reaction, no activity with wild-type enzyme Methanothermobacter thermautotrophicus AMP + diphosphate + heptanoyl-CoA
-
 ?
6.2.1.1 ATP + hexanoate + CoA mutant enzyme W416G catalyzes the reaction, no activity with wild-type enzyme Methanothermobacter thermautotrophicus AMP + diphosphate + hexanoyl-CoA
-
 ?
6.2.1.1 ATP + hexanoate + CoA mutant enzyme W416G catalyzes the reaction, no activity with wild-type enzyme Methanothermobacter thermautotrophicus Z245 AMP + diphosphate + hexanoyl-CoA
-
 ?
6.2.1.1 ATP + octanoate + CoA mutant enzyme W416G catalyzes the reaction, no activity with wild-type enzyme Methanothermobacter thermautotrophicus AMP + diphosphate + octanoyl-CoA
-
 ?
6.2.1.1 ATP + propionate + CoA
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + propionyl-CoA
-
 ?
6.2.1.1 ATP + propionate + CoA
-
 Methanothermobacter thermautotrophicus Z245 AMP + diphosphate + propionyl-CoA
-
 ?
6.2.1.1 ATP + valerate + CoA mutant enzyme W416G catalyzes the reaction, no activity with wild-type enzyme Methanothermobacter thermautotrophicus AMP + diphosphate + valeryl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.1 MT-ACS1
-
 Methanothermobacter thermautotrophicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.1 0.67
-
 2-methylvalerate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 1.56
-
 Butyrate 65°C, mutant enzyme V388A Methanothermobacter thermautotrophicus
6.2.1.1 1.9
-
 acetate 65°C, mutant enzyme T313K Methanothermobacter thermautotrophicus
6.2.1.1 3.3
-
 Octanoate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 3.4
-
 Butyrate 65°C, mutant enzyme T313V Methanothermobacter thermautotrophicus
6.2.1.1 5.9
-
 propionate 65°C, mutant enzyme T313V Methanothermobacter thermautotrophicus
6.2.1.1 5.9
-
 hexanoate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 6
-
 propionate 65°C, mutant enzyme V388G Methanothermobacter thermautotrophicus
6.2.1.1 7.3
-
 Heptanoate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 10.8
-
 Butyrate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 10.9
-
 4-methylvalerate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 11.9
-
 valerate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 13.2
-
 propionate 65°C, mutant enzyme V388A Methanothermobacter thermautotrophicus
6.2.1.1 19.2
-
 propionate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 30.4
-
 acetate 65°C, mutant enzyme I312A Methanothermobacter thermautotrophicus
6.2.1.1 31.9
-
 acetate 65°C, mutant enzyme V388G Methanothermobacter thermautotrophicus
6.2.1.1 33.7
-
 acetate 65°C, mutant enzyme W416G Methanothermobacter thermautotrophicus
6.2.1.1 36.2
-
 acetate 65°C, mutant enzyme V388A Methanothermobacter thermautotrophicus
6.2.1.1 46.3
-
 propionate 65°C, wild-type enzyme Methanothermobacter thermautotrophicus
6.2.1.1 65.4
-
 acetate 65°C, wild-type enzyme Methanothermobacter thermautotrophicus