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Literature summary extracted from

  • Pavon, J.A.; Fitzpatrick, P.F.
    Insights into the catalytic mechanisms of phenylalanine and tryptophan hydroxylase from kinetic isotope effects on aromatic hydroxylation (2006), Biochemistry, 45, 11030-11037.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.1 expression of catalytic core mutant DELTA 117PheH and point mutants thereof in Escherichia coli strain C41(DE3) Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.16.1 E280A site-directed mutagenesis of catalytic core mutant DELTA117PheH, 70% reduced activity but unaltered isotopic effects of isotope substrates Rattus norvegicus
1.14.16.1 F263A site-directed mutagenesis of catalytic core mutant DELTA117PheH, 85% reduced activity but unaltered isotopic effects of isotope substrates Rattus norvegicus
1.14.16.1 additional information construction and catalytic properties of deletion mutant DELTA117PheH, consisting of the catalytic core of the enzyme, the isotopic effects of substrates [4-2H]-, [3,5-2H2]-, and 2H5-phenylalanine are unaltered compared to the wild-type enzyme, overview Rattus norvegicus
1.14.16.1 V379D site-directed mutagenesis of catalytic core mutant DELTA117PheH, the isotopic effects of substrates [4-2H]-, [3,5-2H2]-, and 2H5-phenylalanine are altered compared to the wild-type enzyme, overview Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.1 0.063
-
 6-methyl-tetrahydrobiopterin pH 7.0, 25°C, recombinant mutant DELTA 117PheH V379D Rattus norvegicus
1.14.16.1 0.083
-
 6-methyl-tetrahydrobiopterin pH 7.0, 25°C, recombinant mutant DELTA 117PheH Rattus norvegicus
1.14.16.1 0.12
-
 L-phenylalanine pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH Rattus norvegicus
1.14.16.1 0.42
-
 L-phenylalanine pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH V379D Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.1 Fe2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.16.1 L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2 Rattus norvegicus
-
 L-tyrosine + 4a-hydroxy-tetrahydrobiopterin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.1 recombinant catalytic core mutant DELTA 117PheH and point mutants thereof from Escherichia coli strain C41(DE3) Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.16.1 L-phenylalanine + a 5,6,7,8-tetrahydropteridine + O2 = L-tyrosine + a 4a-hydroxy-5,6,7,8-tetrahydropteridine catalytic mechanism Rattus norvegicus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.1 L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2
-
 Rattus norvegicus L-tyrosine + 4a-hydroxy-tetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2 specific for the L-isomer, no activity with the D-isomer, effects of isotopic substrates [4-2H]-, [3,5-2H2]-, and 2H5-phenylalanine, overview Rattus norvegicus L-tyrosine + 4a-hydroxy-tetrahydrobiopterin
-
 ?
1.14.16.1 L-phenylalanine + 6-methyl-tetrahydrobiopterin + O2
-
 Rattus norvegicus L-tyrosine + 6-methyl-4-hydroxy-tetrahydrobiopterin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.16.1 PheH
-
 Rattus norvegicus
1.14.16.1 phenylalanine hydroxylase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.16.1 25
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.16.1 0.031 0.51 6-methyl-tetrahydrobiopterin pH 7.0, 25°C, recombinant mutant DELTA 117PheH Rattus norvegicus
1.14.16.1 0.04 1.97 L-phenylalanine pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH Rattus norvegicus
1.14.16.1 0.3
-
 L-phenylalanine pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH V379D Rattus norvegicus
1.14.16.1 1.12
-
 6-methyl-tetrahydrobiopterin pH 7.0, 25°C, recombinant mutant DELTA 117PheH V379D Rattus norvegicus
1.14.16.1 7.85
-
 6-methyl-tetrahydrobiopterin pH 7.0, 25°C, recombinant mutant DELTA 117PheH Rattus norvegicus
1.14.16.1 8.55
-
 L-phenylalanine pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.16.1 7
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.16.1 5,6,7,8-tetrahydrobiopterin
-
 Rattus norvegicus
1.14.16.1 6-methyl-tetrahydrobiopterin
-
 Rattus norvegicus