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Literature summary extracted from

  • Tarasev, M.; Pinto, A.; Kim, D.; Elliott, S.J.; Ballou, D.P.
    The bridging aspartate 178 in phthalate dioxygenase facilitates interactions between the Rieske center and the iron(II)--mononuclear center (2006), Biochemistry, 45, 10208-10216.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.7 D178A mutation affects protonation of the Rieske center histidine and conformation of subunits within the PDO multimer to create a more open structure with more solvent-accessible Rieske centers Burkholderia cepacia
1.14.12.7 D178N mutation affects protonation of the Rieske center histidine and conformation of subunits within the PDO multimer to create a more open structure with more solvent-accessible Rieske centers Burkholderia cepacia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.7 Fe contains 2.85 Fe per monomer Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.7 48000
-
 6 * 48000, gas phase electrophoretic mobility molecular analysis and electrospray ionization mass spectrometry Burkholderia cepacia
1.14.12.7 290000
-
 SDS-PAGE Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.7 Burkholderia cepacia
-
-
-
1.14.12.7 Burkholderia cepacia DB01
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.7 phthalate + NADH + O2
-
 Burkholderia cepacia cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O
-
 ?
1.14.12.7 phthalate + NADH + O2
-
 Burkholderia cepacia DB01 cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.7 hexamer 6 * 48000, gas phase electrophoretic mobility molecular analysis and electrospray ionization mass spectrometry Burkholderia cepacia

Synonyms

EC Number Synonyms Comment Organism
1.14.12.7 PDO
-
 Burkholderia cepacia
1.14.12.7 phthalate dioxygenase
-
 Burkholderia cepacia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.12.7 60
-
 thermally stable at 60°C Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.7 NADH
-
 Burkholderia cepacia