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Literature summary extracted from

  • Jiang, Y.; Chan, C.H.; Cronan, J.E.
    The soluble acyl-acyl carrier protein synthetase of Vibrio harveyi B392 is a member of the medium chain acyl-CoA synthetase family (2006), Biochemistry, 45, 10008-10019.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.20 expressed in Escherichia coli strain BL21(DELTADE3) Vibrio harveyi

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.20 additional information aasS::kan insertion mutant retains low levels of acyl-acyl carrier protein synthetase Vibrio harveyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.20 60400
-
 SDS-PAGE, hexahistidine-tagged Vibrio harveyi

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.20 Vibrio harveyi Q00IB3
-
-
6.2.1.20 Vibrio harveyi B392 Q00IB3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.20 Ni-NTA column chromatography Vibrio harveyi

Storage Stability

EC Number Storage Stability Organism
6.2.1.20 -80°C, 20 mM Tris-HCl (pH 7.5), 10% glycerol, 1 mM EDTA, 0.1 mM dithiothreitol and 0.002% Triton X-100, several months, no detectable loss of activity Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.20 ATP + an acid + [acyl-carrier protein]
-
 Vibrio harveyi AMP + diphosphate + acyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + an acid + [acyl-carrier protein]
-
 Vibrio harveyi B392 AMP + diphosphate + acyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + octanoate + [acyl-carrier protein] acyl-carrier protein from Eschrichia coli is a good substrate, whilst octanoylation of the Lactococcus lactis acyl-carrier protein is very weak and that of the bovine mitochondrial acyl-carrier protein only barely detectable Vibrio harveyi AMP + diphosphate + octanoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + octanoate + [acyl-carrier protein] acyl-carrier protein from Eschrichia coli is a good substrate, whilst octanoylation of the Lactococcus lactis acyl-carrier protein is very weak and that of the bovine mitochondrial acyl-carrier protein only barely detectable Vibrio harveyi B392 AMP + diphosphate + octanoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + tetradecanoate + [acyl-carrier protein]
-
 Vibrio harveyi AMP + diphosphate + tetradecanoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + tetradecanoate + [acyl-carrier protein]
-
 Vibrio harveyi B392 AMP + diphosphate + tetradecanoyl-[acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.20 AasS
-
 Vibrio harveyi
6.2.1.20 Acyl-acyl carrier protein synthetase
-
 Vibrio harveyi