Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kao, M.C.; Nakamaru-Ogiso, E.; Matsuno-Yagi, A.; Yagi, T.
    Characterization of the membrane domain subunit NuoK (ND4L) of the NADH-quinone oxidoreductase from Escherichia coli (2005), Biochemistry, 44, 9545-9554.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.2 E36A almost no NDH-1 activity Escherichia coli
7.1.1.2 E36Q almost no NDH-1 activity Escherichia coli
7.1.1.2 E72A NDH-1 activity similar to wild type enzyme Escherichia coli
7.1.1.2 E72Q NDH-1 activity similar to wild type enzyme Escherichia coli
7.1.1.2 F15A NDH-1 activity similar to wild type enzyme Escherichia coli
7.1.1.2 G21V reduced NDH-1 activity Escherichia coli
7.1.1.2 R25A reduced NDH-1 activity Escherichia coli
7.1.1.2 R25A/R26A strongly reduced NDH-1 activity Escherichia coli
7.1.1.2 R25K reduced NDH-1 activity Escherichia coli
7.1.1.2 R26A reduced NDH-1 activity Escherichia coli
7.1.1.2 R26K NDH-1 activity similar to wild type enzyme Escherichia coli
7.1.1.2 R85A NDH-1 activity similar to wild type enzyme Escherichia coli
7.1.1.2 R85K NDH-1 activity similar to wild type enzyme Escherichia coli
7.1.1.2 R87A NDH-1 activity similar to wild type enzyme Escherichia coli
7.1.1.2 R87K NDH-1 activity similar to wild type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.2 capsaicin-40 0.01 mM Escherichia coli
7.1.1.2 N,N'-dicyclohexylcarbodiimide 2 mM, 71% inhibition Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.2 membrane
-
 Escherichia coli 16020
-

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.2 0.04
-
 mutant enzyme E36A, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.042
-
 mutant enzyme E36Q, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.08
-
 mutant enzyme R25A/R26A, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.149
-
 mutant enzyme E72Q, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.151
-
 mutant enzyme R25A, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.176
-
 mutant enzyme R25K, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.222
-
 mutant enzyme R26A, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.273
-
 mutant enzyme E72A, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.351
-
 mutant enzyme G21V, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.514
-
 mutant enzyme F15A, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.556
-
 mutant enzyme R85K, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.564
-
 mutant enzyme R85A, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.568
-
 mutant enzyme R87A, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.571
-
 wild type enzyme, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.603
-
 mutant enzyme R87K, using deamino-NADH as electron donor Escherichia coli
7.1.1.2 0.62
-
 mutant enzyme R26K, using deamino-NADH as electron donor Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 deamino-NADH + dimethoxy-5-methyl-6-decyl-1,4-benzoquinone
-
 Escherichia coli deamino-NAD+ + reduced dimethoxy-5-methyl-6-decyl-1,4-benzoquinone
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.2 More NuoK is the smallest subunit of Escherichia coli NDH-1 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 complex I
-
 Escherichia coli
7.1.1.2 NADH-quinone oxidoreductase
-
 Escherichia coli
7.1.1.2 NDH-1
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.2 deamino-NADH
-
 Escherichia coli
7.1.1.2 NADH
-
 Escherichia coli