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Literature summary extracted from

  • Sinegina, L.; Wikstroem, M.; Verkhovsky, M.I.; Verkhovskaya, M.L.
    Activation of isolated NADH:ubiquinone reductase I (complex I) from Escherichia coli by detergent and phospholipids. Recovery of ubiquinone reductase activity and changes in EPR signals of iron-sulfur clusters (2005), Biochemistry, 44, 8500-8506.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.1.1.2 asolectin
-
 Escherichia coli
7.1.1.2 dodecyl maltoside strong stimulation of activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.2 rolliniastatin strong inhibitor Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.2 [4Fe-4S] center
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Escherichia coli
-
-
-
7.1.1.2 Escherichia coli MWC215
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.2 DEAE-Trisacryl M anion exchange column chromatography, Source 15Q column chromatography and ultracentrifugation Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.2 2.2
-
 membrane extract, using hexaammineruthenium-III-chloride as substrate Escherichia coli
7.1.1.2 90
-
 after purification, using hexaammineruthenium-III-chloride as substrate Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 NADH + decylubiquinone
-
 Escherichia coli NAD+ + decylubiquinol
-
 ?
7.1.1.2 NADH + decylubiquinone
-
 Escherichia coli MWC215 NAD+ + decylubiquinol
-
 ?
7.1.1.2 NADH + hexaammineruthenium-III-chloride
-
 Escherichia coli NAD+ + ?
-
 ?
7.1.1.2 NADH + hexaammineruthenium-III-chloride
-
 Escherichia coli MWC215 NAD+ + ?
-
 ?
7.1.1.2 NADH + ubiquinone
-
 Escherichia coli NAD+ + ubiquinol
-
 ?
7.1.1.2 NADH + ubiquinone
-
 Escherichia coli MWC215 NAD+ + ubiquinol
-
 ?
7.1.1.2 NADH + ubiquinone-8
-
 Escherichia coli NAD+ + ubiquinol-8
-
 ?
7.1.1.2 NADH + ubiquinone-8
-
 Escherichia coli MWC215 NAD+ + ubiquinol-8
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 complex I
-
 Escherichia coli
7.1.1.2 NADH:ubiquinone oxidoreductase
-
 Escherichia coli
7.1.1.2 NDH-1
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.2 1500
-
 hexaammineruthenium-III-chloride
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.2 FMN
-
 Escherichia coli
7.1.1.2 NADH
-
 Escherichia coli