Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fox, D.T.; Poulter, C.D.
    Mechanistic studies with 2-C-methyl-D-erythritol 4-phosphate synthase from Escherichia coli (2005), Biochemistry, 44, 8360-8368.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.267 overexpression in strain BL21 as His-tagged enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.267 additional information 1,1,1-trifluoro-1-deoxy-D-xylulose 5-phosphoric acid, 1,1-difluoro-1-deoxy-D-xylulose 5-phosphoric acid, and 1,2-dideoxy-D-hexulose 6-phosphate are poor inhibitors, most likely because of the increase in steric bulk at C1 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.267 additional information
-
 additional information steady-state and single-turnover kinetics, primary kinetic isotope effects, overview Escherichia coli
1.1.1.267 0.0005
-
 NADPH pH 7.6, 37°C, recombinant enzyme Escherichia coli
1.1.1.267 0.045
-
 1-deoxy-D-xylulose 5-phosphate pH 7.6, 37°C, recombinant enzyme Escherichia coli
1.1.1.267 0.227
-
 1-fluoro-1-deoxy-D-xylulose 5-phosphate pH 7.6, 37°C, recombinant enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.267 Mg2+ activates Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Escherichia coli
-
 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.267 recombinant His-tagged enzyme from overexpressing strain BL21 by nickel affinity chromatography Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.267 2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ the reaction does not exhibit burst kinetics for either substrate, indicating that product release is not rate-limiting, the rearrangement step, which precedes hydride transfer, is rate-limiting for 1-deoxy-D-xylulose 5-phosphate but becomes partially ratelimiting for 1-fluoro-1-deoxy-D-xylulose 5-phosphate, thus, rearrangement appears to be enhanced by substitution of a hydrogen atom in the methyl group of 1-deoxy-D-xylulose 5-phosphate by fluorine, consistent with a retro-aldol/aldol mechanism for the rearrangement during conversion of 1-deoxy-D-xylulose 5-phosphate to 2-C-methyl-D-erythritol 4-phosphate Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.267 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
 Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 r
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ i.e. DXP Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 r
1.1.1.267 1-fluoro-1-deoxy-D-xylulose 5-phosphate + NADPH i.e. CF-DXP, results in about 25% of the activity with 1-deoxy-D-xylulose 5-phosphate Escherichia coli ?
-
 r
1.1.1.267 additional information 1,1,1-trifluoro-1-deoxy-D-xylulose 5-phosphoric acid, 1,1-difluoro-1-deoxy-D-xylulose 5-phosphoric acid, and 1,2-dideoxy-D-hexulose 6-phosphate are poor substrates Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 2-C-methyl-D-erythritol 4-phosphate synthase
-
 Escherichia coli
1.1.1.267 MEP synthase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.267 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.267 29
-
 1-deoxy-D-xylulose 5-phosphate pH 7.6, 37°C, recombinant enzyme Escherichia coli
1.1.1.267 37
-
 1-fluoro-1-deoxy-D-xylulose 5-phosphate pH 7.6, 37°C, recombinant enzyme Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.267 7.6
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADP+
-
 Escherichia coli
1.1.1.267 NADPH
-
 Escherichia coli