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Literature summary extracted from

  • Ho, K.K.; Allali-Hassani, A.; Hurley, T.D.; Weiner, H.
    Differential effects of Mg2+ ions on the individual kinetic steps of human cytosolic and mitochondrial aldehyde dehydrogenases (2005), Biochemistry, 44, 8022-8029.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.3 E399Q mutant is not inhibited by MgCl2 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 Mg2+ 85% inhibition at 0.5 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.0002
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25°C Homo sapiens
1.2.1.3 0.0002
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C Homo sapiens
1.2.1.3 0.0003
-
 NAD+ 50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,0.14 mM propanal, 25°C Homo sapiens
1.2.1.3 0.0012
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25°C Homo sapiens
1.2.1.3 0.0052
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C Homo sapiens
1.2.1.3 0.0062
-
 NAD+ 50 mM sodium phosphate buffer, pH 7.4, 0.14 mM propanal, 25°C Homo sapiens
1.2.1.3 0.041
-
 NAD+ 50 mM sodium phosphate buffer, pH 7.4, 0.014 mM propanal, 25°C Homo sapiens
1.2.1.3 0.12
-
 NAD+ 50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,0.014 mM propanal, 25°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.3 Mg2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Homo sapiens P05091
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 two chromatography steps, homogeneous on SDS-PAGE Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.3 cell culture
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Homo sapiens acetate + NADH + H+
-
 ?
1.2.1.3 benzaldehyde + NAD+ + H2O
-
 Homo sapiens benzoate + NADH + H+
-
 ?
1.2.1.3 propanal + NAD+ + H2O
-
 Homo sapiens propionate + NADH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 ALDH1
-
 Homo sapiens
1.2.1.3 ALDH2
-
 Homo sapiens
1.2.1.3 class 1 aldehyde dehydrogenase
-
 Homo sapiens
1.2.1.3 class 2 aldehyde dehydrogenase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.3 additional information
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25°C Homo sapiens
1.2.1.3 0.65
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25°C Homo sapiens
1.2.1.3 1.33
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C Homo sapiens
1.2.1.3 3.16
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C Homo sapiens
1.2.1.3 6.16
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Homo sapiens