Literature summary extracted from
Zhao, G.; Jorns, M.S.
Ionization of zwitterionic amine substrates bound to monomeric sarcosine oxidase (2005), Biochemistry, 44, 16866-16874.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.5.3.1 |
Y317F |
20fold decrease in the maxumim rate of the reductive half-reaction. Unlike wild-type, Kd-values of mutant are pH-dependent |
Bacillus sp. (in: Bacteria) |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.5.3.1 |
methylthioacetate |
comparison of complexes with wild-type and mutant Y317F |
Bacillus sp. (in: Bacteria) |
|
1.5.3.1 |
pyrrole-2-carboxylate |
comparison of complexes with wild-type and mutant Y317F |
Bacillus sp. (in: Bacteria) |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.5.3.1 |
Bacillus sp. (in: Bacteria) |
- |
- |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
1.5.3.1 |
sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 |
L-proline is the ionizable group in the ES complex. Y317 may play a role in substrate activation and optimizing of binding |
Bacillus sp. (in: Bacteria) |
|