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Literature summary extracted from

  • Magalhaes, M.L.; Blanchard, J.S.
    The kinetic mechanism of AAC3-IV aminoglycoside acetyltransferase from Escherichia coli (2005), Biochemistry, 44, 16275-16283.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.81 expressed in the pET-23a(+) expression vector Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.81 desulfo-CoA
-
 Escherichia coli
2.3.1.81 netilmicin
-
 Escherichia coli
2.3.1.81 ribostamycin
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.81 0.00015
-
 paromomycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 0.00033
-
 sisomycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 0.00036
-
 neomycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 0.00067
-
 apramycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 0.00097
-
 gentamycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 0.00142
-
 tobramycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 0.0031
-
 ribostamycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 0.042
-
 netilmicin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 0.086
-
 acetyl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 0.237
-
 butirosin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 0.238
-
 kanamycin A in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 0.477
-
 malonyl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 0.499
-
 propionyl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 1.16
-
 butyryl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 11.07
-
 amikacin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.81 28000
-
 2 * 28000, enzyme in solution Escherichia coli
2.3.1.81 54000
-
 dynamic light scattering Escherichia coli
2.3.1.81 55750
-
 calculated from amino acid sequence Escherichia coli
2.3.1.81 60000
-
 Sephacryl-S200 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.81 Escherichia coli Q306W4
-
-
2.3.1.81 Escherichia coli BL21(DE3) pLysS Q306W4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.81 Fast Flow Q-Sepharose anion exchange column chromatography and Phenyl Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.81 acetyl-CoA + amikacin
-
 Escherichia coli CoA + N3'-acetylamikacin
-
 ?
2.3.1.81 acetyl-CoA + amikacin
-
 Escherichia coli BL21(DE3) pLysS CoA + N3'-acetylamikacin
-
 ?
2.3.1.81 acetyl-CoA + apramycin
-
 Escherichia coli CoA + N3'-acetylapramycin
-
 ?
2.3.1.81 acetyl-CoA + apramycin
-
 Escherichia coli BL21(DE3) pLysS CoA + N3'-acetylapramycin
-
 ?
2.3.1.81 acetyl-CoA + butirosin
-
 Escherichia coli CoA + N3'-acetylbutirosin
-
 ?
2.3.1.81 acetyl-CoA + gentamicin
-
 Escherichia coli CoA + N3'-acetylgentamicin
-
 ?
2.3.1.81 acetyl-CoA + gentamicin
-
 Escherichia coli BL21(DE3) pLysS CoA + N3'-acetylgentamicin
-
 ?
2.3.1.81 acetyl-CoA + kanamycin
-
 Escherichia coli CoA + N3'-acetylkanamycin
-
 ?
2.3.1.81 acetyl-CoA + kanamycin
-
 Escherichia coli BL21(DE3) pLysS CoA + N3'-acetylkanamycin
-
 ?
2.3.1.81 acetyl-CoA + neomycin
-
 Escherichia coli CoA + ?
-
 ?
2.3.1.81 acetyl-CoA + netilmicin
-
 Escherichia coli CoA + N3'-acetylnetilmicin
-
 ?
2.3.1.81 acetyl-CoA + paromomycin
-
 Escherichia coli CoA + N3'-acetylparomomycin
-
 ?
2.3.1.81 acetyl-CoA + ribostamycin
-
 Escherichia coli CoA + ?
-
 ?
2.3.1.81 acetyl-CoA + sisomycin
-
 Escherichia coli CoA + N3'-acetylsisomycin
-
 ?
2.3.1.81 acetyl-CoA + tobramycin
-
 Escherichia coli CoA + N3'-acetyltobramycin
-
 ?
2.3.1.81 acetyl-CoA + tobramycin
-
 Escherichia coli BL21(DE3) pLysS CoA + N3'-acetyltobramycin
-
 ?
2.3.1.81 butyryl-CoA + ribostamycin
-
 Escherichia coli CoA + ?
-
 ?
2.3.1.81 desulfo-CoA + ribostamycin
-
 Escherichia coli ?
-
 ?
2.3.1.81 malonyl-CoA + ribostamycin
-
 Escherichia coli CoA + ?
-
 ?
2.3.1.81 propionyl-CoA + ribostamycin
-
 Escherichia coli CoA + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.81 dimer 2 * 28000, enzyme in solution Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.3.1.81 AAC(3)-IV
-
 Escherichia coli
2.3.1.81 AAC3-IV aminoglycoside acetyltransferase
-
 Escherichia coli
2.3.1.81 aminoglycoside 3-N-acetyltransferase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.81 0.3
-
 butyryl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 0.5
-
 amikacin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 3.5
-
 butirosin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 9
-
 malonyl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 15
-
 paromomycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 32
-
 netilmicin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 33
-
 propionyl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 39
-
 acetyl-CoA in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 40
-
 gentamycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 51
-
 sisomycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 60
-
 neomycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 61
-
 apramycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 80
-
 kanamycin A in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 146
-
 ribostamycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli
2.3.1.81 162
-
 tobramycin in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.81 8
-
 corresponds to the plateau region of the pH profile, where the velocity is maximal and the variations in velocity due to changes in pH are smallest Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.81 Escherichia coli calculated from amino acid sequence
-
 5.7