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Literature summary extracted from

  • Itaeaho, K.; Alakurtti, S.; Yli-Kauhaluoma, J.; Taskinen, J.; Coughtrie, M.W.; Kostiainen, R.
    Regioselective sulfonation of dopamine by SULT1A3 in vitro provides a molecular explanation for the preponderance of dopamine-3-O-sulfate in human blood circulation (2007), Biochem. Pharmacol., 74, 504-510.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.1 SULT1A3, expression in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.1 dopamine 3-O-sulfate 35-85% product inhibition of SULT1A3 Homo sapiens
2.8.2.1 dopamine 4-O-sulfate 40-80% product inhibition of SULT1A3 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.1 additional information
-
 additional information SULT1A3, kinetics Homo sapiens
2.8.2.1 0.0022
-
 dopamine dopamine 4-O-sulfate formation, recombinant SULT1A3 Homo sapiens
2.8.2.1 0.0026
-
 dopamine dopamine 3-O-sulfate formation, recombinant SULT1A3 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.1 2 3'-phosphoadenylyl sulfate + 2 dopamine Homo sapiens SULT1A3 2 adenosine 3',5'-bisphosphate + dopamine 3-O-sulfate + dopamine 4-O-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.1 Homo sapiens P0DMM9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.1 recombinant SULT1A3 from Escherichia coli strain by ammonium sulfate fractionation, anion exchange and adenosine 3',5'-bisphosphate affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.1 additional information
-
 development of a HPLC-based dopamine 3-O-sulfate and dopamine 4-O-sulfate product detection method, the formation of the dopamine 3-O-sulfate is much higher with SULT1A3 than the dopamine 4-O-sulfate formation, overview Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.1 2 3'-phosphoadenylyl sulfate + 2 dopamine SULT1A3 Homo sapiens 2 adenosine 3',5'-bisphosphate + dopamine 3-O-sulfate + dopamine 4-O-sulfate
-
 ?
2.8.2.1 2 3'-phosphoadenylyl sulfate + 2 dopamine SULT1A3 Homo sapiens 2 adenosine 3',5'-bisphosphate + dopamine 3-O-sulfate + dopamine 4-O-sulfate NMR product structure analysis, dopamine 3-O-sulfate is the main product, overview ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.1 SULT1A3
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.1 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.1 6.8
-
 assay at Homo sapiens