EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.5.2 | - |
Plasmodium falciparum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.5.2 | 6-thioguanine | 0.5 mM, 27% inhibition | Plasmodium falciparum | |
6.3.5.2 | 8-Azaguanine | 0.5 mM, 40% inhibition | Plasmodium falciparum | |
6.3.5.2 | adenosine | 0.5 mM, 37% inhibition | Plasmodium falciparum | |
6.3.5.2 | adenosine 5'-(beta,gamma-imido)triphosphate | non-competitive versus XMP, competitive versus ATP | Plasmodium falciparum | |
6.3.5.2 | AMP | 0.5 mM, 22% inhibitiion; 0.5 mM, 22% inhibition | Plasmodium falciparum | |
6.3.5.2 | AMP-PNP | potent inhibitor, competitive and non-competitive inhibitor with respect to ATP and XMP | Plasmodium falciparum | |
6.3.5.2 | diphosphate | non-competitive versus XMP, competitive versus ATP, non-competitive versus L-Gln; potent inhibitor, competitive and non-competitive inhibition with respect to ATP and XMP | Plasmodium falciparum | |
6.3.5.2 | GDP | 0.5 mM, 42% inhibition | Plasmodium falciparum | |
6.3.5.2 | GMP | competitive versus XMP, uncompetitive versus ATP, non-competitive versus L-Gln; potent inhibitor, competitive and uncompetitive inhibition with respect to XMP and ATP | Plasmodium falciparum | |
6.3.5.2 | GTP | 0.5 mM, 40% inhibition | Plasmodium falciparum | |
6.3.5.2 | guanosine | 0.5 mM, 50% inhibition | Plasmodium falciparum | |
6.3.5.2 | IMP | 0.5 mM, 10% inhibition | Plasmodium falciparum | |
6.3.5.2 | K+ | - |
Plasmodium falciparum | |
6.3.5.2 | Li+ | - |
Plasmodium falciparum | |
6.3.5.2 | additional information | not inhibitory at 0.5 mM: hypoxanthine, chloroadenosine, xanthosine, inosine, decoyinine | Plasmodium falciparum | |
6.3.5.2 | Na+ | 25% loss of activity at 24 mM, 90% loss of activity at 200 mM | Plasmodium falciparum | |
6.3.5.2 | phosphate | - |
Plasmodium falciparum | |
6.3.5.2 | Psicofuranine | 0.5 mM, 25% inhibition | Plasmodium falciparum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.5.2 | 0.0168 | - |
XMP | pH 8.5, 37°C | Plasmodium falciparum | |
6.3.5.2 | 0.0168 | - |
XMP | 37°C, pH 8.5 | Plasmodium falciparum | |
6.3.5.2 | 0.26 | - |
ATP | pH 8.5, 37°C | Plasmodium falciparum | |
6.3.5.2 | 0.26 | - |
ATP | 37°C, pH 8.5 | Plasmodium falciparum | |
6.3.5.2 | 0.472 | - |
L-Gln | pH 8.5, 37°C | Plasmodium falciparum | |
6.3.5.2 | 0.472 | - |
L-glutamine | 37°C, pH 8.5 | Plasmodium falciparum | |
6.3.5.2 | 5.4 | - |
NH4+ | 37°C, pH 8.5 | Plasmodium falciparum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.5.2 | Ca2+ | replacement of Mg2+ with Mn2+ leads to 85% drop in activity | Plasmodium falciparum | |
6.3.5.2 | Mg2+ | required for catalysis | Plasmodium falciparum | |
6.3.5.2 | Mg2+ | half-maximal activity at 2.1 mM, Hill-coefficient of 4.4 | Plasmodium falciparum | |
6.3.5.2 | Mn2+ | replacement of Mg2+ with Mn2+ leads to 70% drop in activity | Plasmodium falciparum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.5.2 | 65000 | - |
x * 65000, SDS-PAGE | Plasmodium falciparum |
6.3.5.2 | 160000 | - |
gel filtration | Plasmodium falciparum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.5.2 | Plasmodium falciparum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.5.2 | - |
Plasmodium falciparum |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.3.5.2 | ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate | steady-state ordered binding of ATP followed by XMP to the ATPase domain with glutamine binding in a random manner to the GAT domain | Plasmodium falciparum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.5.2 | ATP + xanthosine 5'-phosphate + L-glutamine + H2O | steady state ordered binding of the substrates ATP and XMP to the ATPase domain, with ATP being the first substrate to bind followed by XMP | Plasmodium falciparum | AMP + diphosphate + GMP + L-glutamate | - |
? | |
6.3.5.2 | ATP + XMP + L-Gln | - |
Plasmodium falciparum | AMP + diphosphate + GMP + L-Glu | - |
? | |
6.3.5.2 | ATP + XMP + NH4+ | NH4+ in form of (NH4)2SO4 | Plasmodium falciparum | AMP + diphosphate + GMP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.5.2 | ? | x * 65000, SDS-PAGE | Plasmodium falciparum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.5.2 | GMPS | - |
Plasmodium falciparum |
6.3.5.2 | Guanosine 5-monophosphate synthetase | - |
Plasmodium falciparum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.5.2 | 0.43 | - |
XMP | pH 8.5, 37°C | Plasmodium falciparum | |
6.3.5.2 | 0.43 | - |
ATP | 37°C, pH 8.5 | Plasmodium falciparum | |
6.3.5.2 | 0.43 | - |
L-glutamine | 37°C, pH 8.5 | Plasmodium falciparum | |
6.3.5.2 | 0.43 | - |
XMP | 37°C, pH 8.5 | Plasmodium falciparum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.5.2 | 0.0364 | - |
diphosphate | 37°C, pH 8.5, with ATP as variable substrate | Plasmodium falciparum | |
6.3.5.2 | 0.0364 | - |
diphosphate | substrate ATP, pH 8.5, 37°C | Plasmodium falciparum | |
6.3.5.2 | 0.038 | - |
GMP | 37°C, pH 8.5, with XMP as variable substrate | Plasmodium falciparum | |
6.3.5.2 | 0.038 | - |
GMP | substrate XMP, pH 8.5, 37°C | Plasmodium falciparum | |
6.3.5.2 | 0.179 | - |
GMP | 37°C, pH 8.5, with ATP as variable substrate | Plasmodium falciparum | |
6.3.5.2 | 0.179 | - |
GMP | substrate ATP, pH 8.5, 37°C | Plasmodium falciparum | |
6.3.5.2 | 0.21 | - |
AMP-PNP | 37°C, pH 8.5, with ATP as variable substrate | Plasmodium falciparum | |
6.3.5.2 | 0.21 | - |
adenosine 5'-(beta,gamma-imido)triphosphate | substrate ATP, pH 8.5, 37°C | Plasmodium falciparum | |
6.3.5.2 | 0.39 | - |
GMP | 37°C, pH 8.5, with glutamine as variable substrate | Plasmodium falciparum | |
6.3.5.2 | 0.39 | - |
GMP | substrate L-Gln, pH 8.5, 37°C | Plasmodium falciparum | |
6.3.5.2 | 0.443 | - |
diphosphate | 37°C, pH 8.5, with XMP as variable substrate | Plasmodium falciparum | |
6.3.5.2 | 0.443 | - |
diphosphate | substrate XMP, pH 8.5, 37°C | Plasmodium falciparum | |
6.3.5.2 | 0.611 | - |
AMP-PNP | 37°C, pH 8.5, with XMP as variable substrate | Plasmodium falciparum | |
6.3.5.2 | 0.611 | - |
adenosine 5'-(beta,gamma-imido)triphosphate | substrate XMP, pH 8.5, 37°C | Plasmodium falciparum | |
6.3.5.2 | 0.933 | - |
diphosphate | substrate L-Gln, pH 8.5, 37°C | Plasmodium falciparum | |
6.3.5.2 | 933 | - |
diphosphate | 37°C, pH 8.5, with glutamine as variable substrate | Plasmodium falciparum |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
6.3.5.2 | 0.5 | - |
37°C, pH 8.5 | Plasmodium falciparum | guanosine | |
6.3.5.2 | 2.7 | - |
37°C, pH 8.5 | Plasmodium falciparum | phosphate |