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Literature summary extracted from

  • Rendic, D.; Klaudiny, J.; Stemmer, U.; Schmidt, J.; Paschinger, K.; Wilson, I.B.
    Towards abolition of immunogenic structures in insect cells: characterization of a honey-bee (Apis mellifera) multi-gene family reveals both an allergy-related core alpha1,3-fucosyltransferase and the first insect Lewis-histo-blood-group-related antigen-synthesizing enzyme (2007), Biochem. J., 402, 603.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.152 expression in Pichia pastoris GS115 cells Apis mellifera
2.4.1.152 expression in Sf9 cells Apis mellifera
2.4.1.152 the Apis mellifera genome encodes three alpha1,3-FucT homologues FucTA, FucTB and FucTC, expressed in yeast and insect cells only FucTA is found to be a core alpha1,3-FucT (EC 2.1.214), FucTC discloses to be the first Lewis-type alpha1,3-FucT (EC 2.4.1.152) to be described in insects, FucTB does not show any activity Apis mellifera
2.4.1.214 expression in Pichia pastoris GS115 cells Apis mellifera
2.4.1.214 expression in Sf9 cells Apis mellifera
2.4.1.214 the Apis mellifera genome encodes three alpha1,3-FucT homologues FucTA, FucTB and FucTC, expressed in yeast and insect cells only FucTA is found to be a core alpha1,3-FucT (EC 2.1.214), FucTC discloses to be the first Lewis-type alpha1,3-FucT (EC 2.4.1.152) to be described in insects, FucTB does not show any activity Apis mellifera

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.152 Cu2+ reduced activity Apis mellifera
2.4.1.152 additional information not inhibited by EDTA Apis mellifera
2.4.1.214 Cu2+ completely abolishes the enzyme’s activity Apis mellifera
2.4.1.214 EDTA completely abolishes the enzyme’s activity Apis mellifera
2.4.1.214 Zn2+ completely abolishes the enzyme’s activity Apis mellifera

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.152 Mg2+ activated by Mg2+ Apis mellifera
2.4.1.152 Mn2+ activated by Mn2+ Apis mellifera
2.4.1.214 Mn2+ strongly activated by Mn2+ Apis mellifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.152 47000
-
calculation from sequence of cDNA Apis mellifera
2.4.1.214 53000
-
calculation from sequence of cDNA Apis mellifera

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.152 Apis mellifera Q05GU1 honey-bee
-
2.4.1.214 Apis mellifera Q05GU3 honey-bee
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.152 brain
-
Apis mellifera
-
2.4.1.152 hypopharynx predominantly expressed Apis mellifera
-
2.4.1.152 larva
-
Apis mellifera
-
2.4.1.152 thorax muscle
-
Apis mellifera
-
2.4.1.152 venom gland predominantly expressed Apis mellifera
-
2.4.1.214 brain predominantly expressed Apis mellifera
-
2.4.1.214 hypopharynx
-
Apis mellifera
-
2.4.1.214 larva
-
Apis mellifera
-
2.4.1.214 thorax muscle
-
Apis mellifera
-
2.4.1.214 venom gland predominantly expressed Apis mellifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.152 GDP-fucose + Galbeta1-4GlcNAcbeta1-2Manalpha1-6(Galbeta1-4GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn
-
Apis mellifera GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-6(Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn
-
?
2.4.1.152 GDP-fucose + Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-PA Lacto-N-neo-tetraose is a Lewis-type FucT substrate Apis mellifera GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc-PA
-
?
2.4.1.152 GDP-fucose + GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-6(GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn
-
Apis mellifera GDP + GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-6(GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn
-
?
2.4.1.214 GDP-fucose + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4(Fucalpha1-6)GlcNAcbeta1-Asn
-
Apis mellifera GDP + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-3)[Fucalpha1-6]GlcNAcbeta1-Asn difucosylated glycan structure ?
2.4.1.214 GDP-fucose + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn
-
Apis mellifera GDP + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4(Fucalpha1-3)GlcNAcbeta1-Asn
-
?
2.4.1.214 additional information substrate specificity of FucTA of Apis mellifera is similar to Drosophila melanogaster FucTA Apis mellifera ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.152 FucTC
-
Apis mellifera
2.4.1.152 LEwis-type alpha1,3-fucosyltransferase
-
Apis mellifera
2.4.1.214 core alpha1,3-fucosyltransferase
-
Apis mellifera
2.4.1.214 FucTA
-
Apis mellifera

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.152 15
-
maximal activity Apis mellifera
2.4.1.214 23
-
-
Apis mellifera

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.152 4 50 temperature range for FucTC is very broad, enzyme shows 90% of maximal activity at 4°C and 40% of maximal activity at 50°C Apis mellifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.152 6.5
-
maximal activity at pH 6.5 or 8.5 Apis mellifera
2.4.1.152 8.5
-
maximal activity at pH 6.5 or 8.5 Apis mellifera

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.152 6.5 9 pH range for FucTC is very broad Apis mellifera
2.4.1.214 7.5 9 enzyme shows maximal activity in mildly alkaline conditions Apis mellifera