EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.152 | expression in Pichia pastoris GS115 cells | Apis mellifera |
2.4.1.152 | expression in Sf9 cells | Apis mellifera |
2.4.1.152 | the Apis mellifera genome encodes three alpha1,3-FucT homologues FucTA, FucTB and FucTC, expressed in yeast and insect cells only FucTA is found to be a core alpha1,3-FucT (EC 2.1.214), FucTC discloses to be the first Lewis-type alpha1,3-FucT (EC 2.4.1.152) to be described in insects, FucTB does not show any activity | Apis mellifera |
2.4.1.214 | expression in Pichia pastoris GS115 cells | Apis mellifera |
2.4.1.214 | expression in Sf9 cells | Apis mellifera |
2.4.1.214 | the Apis mellifera genome encodes three alpha1,3-FucT homologues FucTA, FucTB and FucTC, expressed in yeast and insect cells only FucTA is found to be a core alpha1,3-FucT (EC 2.1.214), FucTC discloses to be the first Lewis-type alpha1,3-FucT (EC 2.4.1.152) to be described in insects, FucTB does not show any activity | Apis mellifera |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.152 | Cu2+ | reduced activity | Apis mellifera | |
2.4.1.152 | additional information | not inhibited by EDTA | Apis mellifera | |
2.4.1.214 | Cu2+ | completely abolishes the enzymes activity | Apis mellifera | |
2.4.1.214 | EDTA | completely abolishes the enzymes activity | Apis mellifera | |
2.4.1.214 | Zn2+ | completely abolishes the enzymes activity | Apis mellifera |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.152 | Mg2+ | activated by Mg2+ | Apis mellifera | |
2.4.1.152 | Mn2+ | activated by Mn2+ | Apis mellifera | |
2.4.1.214 | Mn2+ | strongly activated by Mn2+ | Apis mellifera |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.152 | 47000 | - |
calculation from sequence of cDNA | Apis mellifera |
2.4.1.214 | 53000 | - |
calculation from sequence of cDNA | Apis mellifera |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.152 | Apis mellifera | Q05GU1 | honey-bee | - |
2.4.1.214 | Apis mellifera | Q05GU3 | honey-bee | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.152 | brain | - |
Apis mellifera | - |
2.4.1.152 | hypopharynx | predominantly expressed | Apis mellifera | - |
2.4.1.152 | larva | - |
Apis mellifera | - |
2.4.1.152 | thorax muscle | - |
Apis mellifera | - |
2.4.1.152 | venom gland | predominantly expressed | Apis mellifera | - |
2.4.1.214 | brain | predominantly expressed | Apis mellifera | - |
2.4.1.214 | hypopharynx | - |
Apis mellifera | - |
2.4.1.214 | larva | - |
Apis mellifera | - |
2.4.1.214 | thorax muscle | - |
Apis mellifera | - |
2.4.1.214 | venom gland | predominantly expressed | Apis mellifera | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.152 | GDP-fucose + Galbeta1-4GlcNAcbeta1-2Manalpha1-6(Galbeta1-4GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn | - |
Apis mellifera | GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-6(Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn | - |
? | |
2.4.1.152 | GDP-fucose + Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-PA | Lacto-N-neo-tetraose is a Lewis-type FucT substrate | Apis mellifera | GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc-PA | - |
? | |
2.4.1.152 | GDP-fucose + GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-6(GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn | - |
Apis mellifera | GDP + GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-6(GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn | - |
? | |
2.4.1.214 | GDP-fucose + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4(Fucalpha1-6)GlcNAcbeta1-Asn | - |
Apis mellifera | GDP + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-3)[Fucalpha1-6]GlcNAcbeta1-Asn | difucosylated glycan structure | ? | |
2.4.1.214 | GDP-fucose + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-Asn | - |
Apis mellifera | GDP + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4(Fucalpha1-3)GlcNAcbeta1-Asn | - |
? | |
2.4.1.214 | additional information | substrate specificity of FucTA of Apis mellifera is similar to Drosophila melanogaster FucTA | Apis mellifera | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.152 | FucTC | - |
Apis mellifera |
2.4.1.152 | LEwis-type alpha1,3-fucosyltransferase | - |
Apis mellifera |
2.4.1.214 | core alpha1,3-fucosyltransferase | - |
Apis mellifera |
2.4.1.214 | FucTA | - |
Apis mellifera |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.152 | 15 | - |
maximal activity | Apis mellifera |
2.4.1.214 | 23 | - |
- |
Apis mellifera |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.152 | 4 | 50 | temperature range for FucTC is very broad, enzyme shows 90% of maximal activity at 4°C and 40% of maximal activity at 50°C | Apis mellifera |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.152 | 6.5 | - |
maximal activity at pH 6.5 or 8.5 | Apis mellifera |
2.4.1.152 | 8.5 | - |
maximal activity at pH 6.5 or 8.5 | Apis mellifera |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.152 | 6.5 | 9 | pH range for FucTC is very broad | Apis mellifera |
2.4.1.214 | 7.5 | 9 | enzyme shows maximal activity in mildly alkaline conditions | Apis mellifera |