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Literature summary extracted from

  • Thorndycroft, F.H.; Butland, G.; Richardson, D.J.; Watmough, N.J.
    A new assay for nitric oxide reductase reveals two conserved glutamate residues form the entrance to a proton-conducting channel in the bacterial enzyme (2007), Biochem. J., 401, 111-119.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.5 expressed in Escherichia coli Paracoccus denitrificans

Protein Variants

EC Number Protein Variants Comment Organism
1.7.2.5 E122A substitution leads to an almost complete loss of NOR activity Paracoccus denitrificans
1.7.2.5 E122D substitution mutant retains 83.2% of the activity of the wild-type enzyme Paracoccus denitrificans
1.7.2.5 E122Q substitution mutant retains 1.3% of the activity of the wild-type enzyme Paracoccus denitrificans
1.7.2.5 E125A substitution leads to an almost complete loss of NOR activity Paracoccus denitrificans
1.7.2.5 E125D substitution leads to an almost complete loss of NOR activity Paracoccus denitrificans
1.7.2.5 E125Q substitution mutant retains 13.4% of the activity of the wild-type enzyme Paracoccus denitrificans
1.7.2.5 E198A substitution mutant retains 1.5% of the activity of the wild-type enzyme Paracoccus denitrificans
1.7.2.5 E202A substitution mutant retains 39.3% of the activity of the wild-type enzyme Paracoccus denitrificans
1.7.2.5 E267A substitution mutant retains 3.6% of the activity of the wild-type enzyme Paracoccus denitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.5 0.027
-
 pseudoazurin apparent KM of native enzyme Paracoccus denitrificans
1.7.2.5 0.035
-
 pseudoazurin apparent KM of recombinant enzyme Paracoccus denitrificans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.5 membrane
-
 Paracoccus denitrificans 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.5 nitric oxide + reduced pseudoazurin Paracoccus denitrificans
-
 nitrous oxide + pseudoazurin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.5 Paracoccus denitrificans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.5
-
 Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.5 2 nitric oxide + reduced acceptor
-
 Paracoccus denitrificans nitrous oxide + acceptor + H2O
-
 ?
1.7.2.5 nitric oxide + reduced pseudoazurin
-
 Paracoccus denitrificans nitrous oxide + pseudoazurin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.5 nitric oxide reductase
-
 Paracoccus denitrificans
1.7.2.5 NO reductase
-
 Paracoccus denitrificans
1.7.2.5 NOR
-
 Paracoccus denitrificans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.5 0.88
-
 pseudoazurin mutant E122Q Paracoccus denitrificans
1.7.2.5 1.01
-
 pseudoazurin mutant E198A Paracoccus denitrificans
1.7.2.5 1.27
-
 pseudoazurin mutant E125D Paracoccus denitrificans
1.7.2.5 1.3
-
 pseudoazurin recombinant wild type enzyme Paracoccus denitrificans
1.7.2.5 2.58
-
 pseudoazurin mutant E267A Paracoccus denitrificans
1.7.2.5 5.18
-
 pseudoazurin mutant E125A Paracoccus denitrificans
1.7.2.5 7
-
 pseudoazurin mutant E122D Paracoccus denitrificans
1.7.2.5 9.43
-
 pseudoazurin mutant E125Q Paracoccus denitrificans
1.7.2.5 10.2
-
 pseudoazurin mutant E122A Paracoccus denitrificans
1.7.2.5 15
-
 pseudoazurin native wild type enzyme Paracoccus denitrificans
1.7.2.5 27.67
-
 pseudoazurin mutant E202A Paracoccus denitrificans
1.7.2.5 58.52
-
 pseudoazurin mutant E122D Paracoccus denitrificans
1.7.2.5 70.38
-
 pseudoazurin recombinant wild type enzyme Paracoccus denitrificans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.2.5 6 8.5
-
 Paracoccus denitrificans