Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rovina, P.; Jaritz, M.; Hoefinger, S.; Graf, C.; Devay, P.; Billich, A.; Baumruker, T.; Bornancin, F.
    A critical beta6-beta7 loop in the pleckstrin homology domain of ceramide kinase (2006), Biochem. J., 400, 255-265.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.138 expressed in COS-1 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.138 K68A/K74A/K80A shows wild type activity Homo sapiens
2.7.1.138 K90V/K98V shows partial activity Homo sapiens
2.7.1.138 K90V/R91A shows partial activity Homo sapiens
2.7.1.138 K90V/R91A/R96A/K98V totally devoid of activity Homo sapiens
2.7.1.138 R29A/R33A/R36A shows wild type activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.138 0.042
-
 ATP assay mixture is composed of 0.88 mM ceramide, 1 mM cardiolipin, 1.5% beta-octylglucoside, 0.2 mM diethylenetriaminepenta-acetic acid, 20 mM MOPS, pH 7.2, 50 mM NaCl, 10 mM dithiothreitol, 2 mM EGTA, 3 mM CaCl2 and 0.5 mM ATP, at 30°C Homo sapiens
2.7.1.138 0.052
-
 ATP assay mixture is composed of 0.88 mM ceramide, 1 mM cardiolipin, 1.5% beta-octylglucoside, 0.2 mM diethylenetriaminepenta-acetic acid, 20 mM MOPS, pH 7.2, 50 mM NaCl, 10 mM dithiothreitol, 2 mM EGTA, 3 mM CaCl2 and 0.5 mM ATP, at 30°C Homo sapiens
2.7.1.138 0.194
-
 ceramide assay mixture is composed of 0.88 mM ceramide, 1 mM cardiolipin, 1.5% beta-octylglucoside, 0.2 mM diethylenetriaminepenta-acetic acid, 20 mM MOPS, pH 7.2, 50 mM NaCl, 10 mM dithiothreitol, 2 mM EGTA, 3 mM CaCl2 and 0.5 mM ATP, at 30°C Homo sapiens
2.7.1.138 0.205
-
 ceramide assay mixture is composed of 0.88 mM ceramide, 1 mM cardiolipin, 1.5% beta-octylglucoside, 0.2 mM diethylenetriaminepenta-acetic acid, 20 mM MOPS, pH 7.2, 50 mM NaCl, 10 mM dithiothreitol, 2 mM EGTA, 3 mM CaCl2 and 0.5 mM ATP, at 30°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.138 cytoplasm
-
 Homo sapiens 5737
-
2.7.1.138 Golgi apparatus
-
 Homo sapiens 5794
-
2.7.1.138 membrane
-
 Homo sapiens 16020
-
2.7.1.138 nucleus
-
 Homo sapiens 5634
-
2.7.1.138 vesicle
-
 Homo sapiens 31982
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.138 Homo sapiens Q8TCT0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.138 Sepharose-bead chromatography and Bio-Spin 6 column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.138 brain
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.138 30°C, 50 mM Tris/HCl, pH 7.5, 270 mM sucrose, 1 mM EDTA, 1 mM EGTA, 5 mM tris(hydroxypropyl)phosphine, 1% Triton X-100, 20 min, 50% decrease of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.138 ATP + ceramide
-
 Homo sapiens ADP + ceramide 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.138 CERK
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.138 ATP
-
 Homo sapiens