Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hidaka, M.; Kitaoka, M.; Hayashi, K.; Wakagi, T.; Shoun, H.; Fushinobu, S.
    Structural dissection of the reaction mechanism of cellobiose phosphorylase (2006), Biochem. J., 398, 37-43.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.20 at 25°C using the sitting-drop vapour diffusion method. Cellobiose phosphorylase-SO4 and cellobiose phosphorylase-PO4 structures are determined at 2.0 and 2.1 A resolution to R factor (Rfree) of 17.6% (21.3%) and 18.0% (22%) respectively. The pH values for crystallization are pH 8.2 for cellobiose phosphorylase-PO4 and pH 6.5 for cellobiose phosphorylase-SO4. Cellulomonas gilvus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.20 8 9 1,5-anhydroglucitol enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus
2.4.1.20 71
-
 isomaltose enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus
2.4.1.20 83
-
 gentiobiose enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus
2.4.1.20 93
-
 melibiose enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.20 Cellulomonas gilvus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.20
-
 Cellulomonas gilvus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.20 1,5-anhydroglucitol + glucose-1-phosphate
-
 Cellulomonas gilvus ?
-
 ?
2.4.1.20 gentiobiose + alpha-D-glucose 1-phosphate
-
 Cellulomonas gilvus ?
-
 ?
2.4.1.20 isomaltose + alpha-D-glucose 1-phosphate
-
 Cellulomonas gilvus ?
-
 ?
2.4.1.20 melibiose + alpha-D-glucose 1-phosphate
-
 Cellulomonas gilvus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.20 dimer
-
 Cellulomonas gilvus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.20 Cbp
-
 Cellulomonas gilvus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.20 1.9
-
 isomaltose enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus
2.4.1.20 2.3
-
 melibiose enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus
2.4.1.20 3.5
-
 1,5-anhydroglucitol enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus
2.4.1.20 7.2
-
 gentiobiose enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0) Cellulomonas gilvus